Chemokine receptor 7 contributes to T- and B-cell filtering in ageing bladder, cystitis and bladder cancer.

IF 5.2 2区 医学 Q1 GERIATRICS & GERONTOLOGY
Jiang Zhao, Xing Luo, Chengfei Yang, Xiao Yang, Min Deng, Bishao Sun, Jingzhen Zhu, Zongming Dong, Yangcai Wang, Jia Li, Xingliang Yang, Benyi Li, Xiangwei Wang, Ji Zheng
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Abstract

Background: Research has suggested significant correlations among ageing, immune microenvironment, inflammation and tumours. However, the relationships among ageing, immune microenvironment, cystitis and bladder urothelial carcinoma (BLCA) in the bladder have rarely been reported.

Methods: Bladder single-cell and transcriptomic data from young and old mice were used for immune landscape analysis. Transcriptome, single-cell and The Cancer Genome Atlas Program datasets of BLCA and interstitial cystitis/bladder pain syndrome (IC/BPS) were used to analyse immune cell infiltration and molecular expression. Bladder tissues from mice, IC/BPS and BLCA were collected to validate the results.

Results: Eight types of immune cells (macrophages, B-cells, dendritic cells, T-cells, monocytes, natural killer cells, γδ T-cells and ILC2) were identified in the bladder of mice. Aged mice bladder tissues had a significantly higher number of T-cells, γδ T-cells, ILC2 and B-cells than those in the young group (P < 0.05). Three types of T-cells (NK T-cells, γδ T-cells and naïve T-cells) and three types of B-cells (follicular B-cells, plasma and memory B-cells) were identified in aged mice bladder. Chemokine receptor 7 (CCR7) is highly expressed in aged bladder, IC/BPS and BLCA (P < 0.05). CCR7 is likely to be involved in T- and B-cell infiltration in aged bladder, IC/BPS and BLCA. Interestingly, the high CCR7 expression on BLCA cell membranes was a prognostic protective factor.

Conclusions: In this study, we characterised the expression profiles of immune cells in bladder tissues of aged and young mice and demonstrated that CCR7-mediated T- and B-cell filtration contributes to the development of bladder ageing, IC/BPS and BLCA.

趋化因子受体 7 对老化膀胱、膀胱炎和膀胱癌中的 T 细胞和 B 细胞过滤有促进作用。
背景:研究表明,衰老、免疫微环境、炎症和肿瘤之间存在明显的相关性。然而,有关膀胱衰老、免疫微环境、膀胱炎和膀胱尿路上皮癌(BLCA)之间关系的研究却鲜有报道:方法:利用年轻小鼠和老年小鼠的膀胱单细胞和转录组数据进行免疫景观分析。利用BLCA和间质性膀胱炎/膀胱疼痛综合征(IC/BPS)的转录组、单细胞和癌症基因组图谱计划数据集分析免疫细胞浸润和分子表达。收集了小鼠、IC/BPS 和 BLCA 的膀胱组织来验证结果:结果:在小鼠膀胱中发现了八种类型的免疫细胞(巨噬细胞、B 细胞、树突状细胞、T 细胞、单核细胞、自然杀伤细胞、γδ T 细胞和 ILC2)。老龄小鼠膀胱组织中的 T 细胞、γδ T 细胞、ILC2 和 B 细胞数量明显高于年轻组(P 结论:老龄小鼠膀胱组织中的 T 细胞、γδ T 细胞、ILC2 和 B 细胞数量明显高于年轻组(P):在这项研究中,我们分析了老龄小鼠和年轻小鼠膀胱组织中免疫细胞的表达谱,并证明了 CCR7 介导的 T 细胞和 B 细胞滤过是膀胱老化、IC/BPS 和 BLCA 发展的原因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Immunity & Ageing
Immunity & Ageing GERIATRICS & GERONTOLOGY-IMMUNOLOGY
CiteScore
10.20
自引率
3.80%
发文量
55
期刊介绍: Immunity & Ageing is a specialist open access journal that was first published in 2004. The journal focuses on the impact of ageing on immune systems, the influence of aged immune systems on organismal well-being and longevity, age-associated diseases with immune etiology, and potential immune interventions to increase health span. All articles published in Immunity & Ageing are indexed in the following databases: Biological Abstracts, BIOSIS, CAS, Citebase, DOAJ, Embase, Google Scholar, Journal Citation Reports/Science Edition, OAIster, PubMed, PubMed Central, Science Citation Index Expanded, SCImago, Scopus, SOCOLAR, and Zetoc.
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