HSP70 positively regulates translation by interacting with the IRES and stabilizes the viral structural proteins VP1 and VP3 to facilitate duck hepatitis A virus type 1 replication.

IF 3.7 1区 农林科学 Q1 VETERINARY SCIENCES
Yurui Jiang, Chenxia Xu, Anchun Cheng, Mingshu Wang, Wei Zhang, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Bin Tian, Juan Huang, Xumin Ou, Di Sun, Yu He, Zhen Wu, Dekang Zhu, Renyong Jia, Shun Chen, Mafeng Liu
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引用次数: 0

Abstract

The maintenance of viral protein homeostasis depends on the interaction between host cell proteins and viral proteins. As a molecular chaperone, heat shock protein 70 (HSP70) has been shown to play an important role in viral infection. Our results showed that HSP70 can affect translation, replication, assembly, and release during the life cycle of duck hepatitis A virus type 1 (DHAV-1). We demonstrated that HSP70 can regulate viral translation by interacting with the DHAV-1 internal ribosome entry site (IRES). In addition, HSP70 interacts with the viral capsid proteins VP1 and VP3 and promotes their stability by inhibiting proteasomal degradation, thereby facilitating the assembly of DHAV-1 virions. This study demonstrates the specific role of HSP70 in regulating DHAV-1 replication, which are helpful for understanding the pathogenesis of DHAV-1 infection and provide additional information about the role of HSP70 in infection by different kinds of picornaviruses, as well as the interaction between picornaviruses and host cells.

HSP70 通过与 IRES 相互作用来积极调节翻译,并稳定病毒结构蛋白 VP1 和 VP3,从而促进甲型肝炎病毒 1 型鸭的复制。
病毒蛋白质平衡的维持取决于宿主细胞蛋白质与病毒蛋白质之间的相互作用。作为一种分子伴侣,热休克蛋白 70(HSP70)在病毒感染中发挥着重要作用。我们的研究结果表明,在鸭甲型肝炎病毒 1 型(DHAV-1)的生命周期中,HSP70 可影响其翻译、复制、组装和释放。我们证实,HSP70可通过与DHAV-1内部核糖体进入位点(IRES)相互作用来调控病毒翻译。此外,HSP70 还能与病毒帽蛋白 VP1 和 VP3 相互作用,并通过抑制蛋白酶体降解促进其稳定性,从而促进 DHAV-1 病毒的组装。这项研究证明了HSP70在调控DHAV-1复制中的特殊作用,有助于理解DHAV-1感染的发病机制,并为HSP70在不同类型短小病毒感染中的作用以及短小病毒与宿主细胞之间的相互作用提供了更多信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Veterinary Research
Veterinary Research 农林科学-兽医学
CiteScore
7.00
自引率
4.50%
发文量
92
审稿时长
3 months
期刊介绍: Veterinary Research is an open access journal that publishes high quality and novel research and review articles focusing on all aspects of infectious diseases and host-pathogen interaction in animals.
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