Subminute thermal damage to cell types present in the skin.

IF 3 3区 医学 Q2 ONCOLOGY
International Journal of Hyperthermia Pub Date : 2024-01-01 Epub Date: 2024-05-16 DOI:10.1080/02656736.2024.2354435
Meagan Doppegieter, Ton G van Leeuwen, Angela van Weert, Maurice C G Aalders, Erik N T P Bakker
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Abstract

Introduction: Psoriasis is characterized by an increase in the proliferation of keratinocytes and nerve fiber activity, contributing to the typical skin lesions. Pulsed Dye Laser (PDL) treatment is effective for the treatment of psoriatic lesions but its mechanism remains unclear. One hypothesis is that PDL causes thermal damage by the diffusion of heat to neighboring structures in lesional skin. There is limited information on the thermal sensitivity of these neighboring skin cells when exposed to hyperthermia for durations lasting less than a minute. Our study aimed to investigate the cell-specific responses to heat using sub-minute exposure times and moderate to ablative hyperthermia.

Materials and methods: Cultured human endothelial cells, smooth muscle cells, neuronal cells, and keratinocytes were exposed to various time (2-20 sec) and temperature (45-70 °C) combinations. Cell viability was assessed by measuring intracellular ATP content 24 h after thermal exposure and this data was used to calculate fit parameters for the Arrhenius model and CEM43 calculations.

Results: Our results show significant differences in cell survival between cell types (p < 0.0001). Especially within the range of 50-60 °C, survival of neuronal cells and keratinocytes was significantly less than that of endothelial and smooth muscle cells. No statistically significant difference was found in the lethal dose (LT50) of thermal energy between neuronal cells and keratinocytes. However, CEM43 calculations showed significant differences between all four cell types.

Conclusion: The results imply that there is a cell-type-dependent sensitivity to thermal damage which suggests that neuronal cells and keratinocytes are particularly susceptible to diffusing heat from laser treatment. Damage to these cells may aid in modulating the neuro-inflammatory pathways in psoriasis. These data provide insight into the potential mechanisms of PDL therapy for psoriasis and advance our understanding of how thermal effects may play a role in its effectiveness.

对皮肤细胞造成的亚微量热损伤。
简介银屑病的特点是角质细胞增生和神经纤维活动增加,从而导致典型的皮肤病变。脉冲染料激光(PDL)治疗对治疗银屑病皮损有效,但其机制仍不清楚。一种假设是,脉冲染料激光通过向病变皮肤邻近结构扩散热量而造成热损伤。目前有关这些邻近皮肤细胞在暴露于持续时间少于一分钟的高热时的热敏感性的信息还很有限。我们的研究旨在利用亚分钟暴露时间和中度烧蚀热疗来研究细胞对热的特异性反应:将培养的人内皮细胞、平滑肌细胞、神经细胞和角质细胞暴露于不同的时间(2-20 秒)和温度(45-70 °C)组合中。热暴露 24 小时后,通过测量细胞内 ATP 含量评估细胞存活率,并利用这些数据计算阿伦尼乌斯模型的拟合参数和 CEM43 计算结果:结果表明,不同类型细胞的存活率存在明显差异(p):结果表明,细胞类型对热损伤的敏感性具有依赖性,这表明神经元细胞和角质细胞特别容易受到激光治疗产生的扩散热的影响。对这些细胞造成的损伤可能有助于调节银屑病的神经炎症途径。这些数据让我们深入了解了PDL疗法治疗银屑病的潜在机制,并加深了我们对热效应如何在其疗效中发挥作用的理解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
5.90
自引率
12.90%
发文量
153
审稿时长
6-12 weeks
期刊介绍: The International Journal of Hyperthermia
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