Giardia intestinalis extracellular vesicles induce changes in gene expression in human intestinal epithelial cells in vitro

IF 1.4 4区医学 Q3 PARASITOLOGY

Experimental parasitology Pub Date : 2024-05-15 DOI:10.1016/j.exppara.2024.108788

Dongming Yang , Yingnan Liu , Yupeng Ren , Lili Hao , Xichen Zhang , Hongjun Chen , Jingyi Liu

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Abstract

Giardiasis is a common waterborne zoonotic disease caused by Giardia intestinalis. Upon infection, Giardia releases excretory and secretory products (ESPs) including secreted proteins (SPs) and extracellular vesicles (EVs). Although the interplay between ESPs and intestinal epithelial cells (IECs) has been previously described, the functions of EVs in these interactions and their differences from those of SPs require further exploration. In the present study, EVs and EV-depleted SPs were isolated from Giardia ESPs. Proteomic analyses of isolated SPs and EVs showed 146 and 91 proteins, respectively. Certain unique and enriched proteins have been identified in SPs and EVs. Transcriptome analysis of Caco-2 cells exposed to EVs showed 96 differentially expressed genes (DEGs), with 56 upregulated and 40 downregulated genes. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Set Enrichment Analysis (GSEA) indicated that Caco-2 genes related to metabolic processes, the HIF-1 signaling pathway, and the cAMP signaling pathway were affected. This study provides new insights into host-parasite interactions, highlighting the potential significance of EVs on IECs during infections.

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肠贾第虫细胞外囊泡诱导体外人类肠上皮细胞基因表达的变化。

贾第虫病是一种常见的水传播人畜共患病，由肠道贾第虫引起。贾第虫感染后会释放排泄和分泌产物（ESPs），包括分泌蛋白（SPs）和细胞外囊泡（EVs）。尽管以前已经描述过 ESPs 与肠上皮细胞（IECs）之间的相互作用，但 EVs 在这些相互作用中的功能及其与 SPs 的区别还需要进一步探讨。本研究从贾第虫ESPs中分离出了EVs和EV-depleted SPs。对分离的 SPs 和 EVs 进行的蛋白质组学分析分别发现了 146 和 91 个蛋白质。在SPs和EVs中发现了某些独特的富集蛋白。暴露于 EVs 的 Caco-2 细胞的转录组分析显示有 96 个差异表达基因（DEGs），其中 56 个基因上调，40 个基因下调。基因本体（GO）、京都基因和基因组百科全书（KEGG）以及基因组富集分析（GSEA）表明，与代谢过程、HIF-1 信号通路和 cAMP 信号通路相关的 Caco-2 基因受到了影响。这项研究为宿主与寄生虫之间的相互作用提供了新的见解，突出了在感染过程中EVs对IECs的潜在意义。

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来源期刊

Experimental parasitology 医学-寄生虫学

CiteScore

3.10

自引率

4.80%

发文量

160

审稿时长

3 months

期刊介绍： Experimental Parasitology emphasizes modern approaches to parasitology, including molecular biology and immunology. The journal features original research papers on the physiological, metabolic, immunologic, biochemical, nutritional, and chemotherapeutic aspects of parasites and host-parasite relationships.