Non-selective microbiota reduction after the elicitation of a seaweed's immune response

IF 3.6 4区生物学 Q2 ENVIRONMENTAL SCIENCES

Environmental Microbiology Reports Pub Date : 2024-05-17 DOI:10.1111/1758-2229.13268

Jiasui Li, Mahasweta Saha, Marwan E. Majzoub, Teng Yang, Haiyan Chu, Torsten Thomas, Florian Weinberger, Suhelen Egan

{"title":"Non-selective microbiota reduction after the elicitation of a seaweed's immune response","authors":"Jiasui Li, Mahasweta Saha, Marwan E. Majzoub, Teng Yang, Haiyan Chu, Torsten Thomas, Florian Weinberger, Suhelen Egan","doi":"10.1111/1758-2229.13268","DOIUrl":null,"url":null,"abstract":"<p>Pattern-triggered immunity (PTI) is an integral part of the innate immune system of many eukaryotic hosts, assisting in the defence against pathogen invasions. In plants and animals, PTI exerts a selective pressure on the microbiota that can alter community composition. However, the effect of PTI on the microbiota for non-model hosts, including seaweeds, remains unknown. Using quantitative polymerase chain reaction complemented with 16S rRNA gene and transcript amplicon sequencing, this study profiled the impact that PTI of the red seaweed <i>Gracilaria gracilis</i> has on its microbiota. PTI elicitation with agar oligosaccharides resulted in a significant reduction in the number of bacteria (by >75% within 72 h after treatment). However, the PTI elicitation did not cause any significant difference in the community diversity or structure. These findings demonstrated that PTI can be non-selective, and this might help to maintain a stable microbiota by uniformly reducing bacterial loads.</p>","PeriodicalId":163,"journal":{"name":"Environmental Microbiology Reports","volume":"16 3","pages":""},"PeriodicalIF":3.6000,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11101764/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Microbiology Reports","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/1758-2229.13268","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Pattern-triggered immunity (PTI) is an integral part of the innate immune system of many eukaryotic hosts, assisting in the defence against pathogen invasions. In plants and animals, PTI exerts a selective pressure on the microbiota that can alter community composition. However, the effect of PTI on the microbiota for non-model hosts, including seaweeds, remains unknown. Using quantitative polymerase chain reaction complemented with 16S rRNA gene and transcript amplicon sequencing, this study profiled the impact that PTI of the red seaweed Gracilaria gracilis has on its microbiota. PTI elicitation with agar oligosaccharides resulted in a significant reduction in the number of bacteria (by >75% within 72 h after treatment). However, the PTI elicitation did not cause any significant difference in the community diversity or structure. These findings demonstrated that PTI can be non-selective, and this might help to maintain a stable microbiota by uniformly reducing bacterial loads.

Abstract Image

查看原文本刊更多论文

激发海藻免疫反应后，非选择性微生物群减少。

模式触发免疫（PTI）是许多真核生物宿主先天性免疫系统的一个组成部分，有助于抵御病原体的入侵。在植物和动物中，PTI 对微生物群施加选择性压力，从而改变群落组成。然而，PTI 对包括海藻在内的非模式宿主微生物群的影响仍然未知。本研究利用定量聚合酶链反应辅以 16S rRNA 基因和转录本扩增片段测序，分析了红藻 Gracilaria gracilis 的 PTI 对其微生物群的影响。用琼脂低聚糖诱导 PTI 可显著减少细菌数量（处理后 72 小时内减少 75%以上）。然而，PTI 激发并没有导致群落多样性或结构的显著差异。这些研究结果表明，PTI 可以是非选择性的，这可能有助于通过均匀减少细菌负荷来维持微生物群的稳定。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Environmental Microbiology Reports ENVIRONMENTAL SCIENCES-MICROBIOLOGY

CiteScore

6.00

自引率

3.00%

发文量

审稿时长

3.0 months

期刊介绍： The journal is identical in scope to Environmental Microbiology, shares the same editorial team and submission site, and will apply the same high level acceptance criteria. The two journals will be mutually supportive and evolve side-by-side. Environmental Microbiology Reports provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens.