Antibiotic susceptibility pattern of Helicobacter pylori against eight antibiotics: A study from North India

IF 4.3 2区医学 Q1 GASTROENTEROLOGY & HEPATOLOGY

Helicobacter Pub Date : 2024-05-16 DOI:10.1111/hel.13093

Safiya Arfi, Prateek Sharma, Mithun Kumar, Shubham Mehra, Kunal Das, Rajashree Das, Ashwini Setya

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Abstract

Background

The purpose of this analysis is to evaluate the antimicrobial susceptibility of eight drugs effective against Helicobacter pylori (H. pylori) strains and the genetic diversity of H. pylori virulence genes to foresee clinical outcomes in North India.

Materials and Methods

Fifty-eight H. pylori strains isolated from patients suffering from various gastrointestinal (GI) diseases were included in the study. MICs of various antibiotics were determined by the agar dilution method. The chi-squared test and Fisher exact test were used to determine the p-value, which was considered significant at p-value ≤ 0.05. RStudio 4.0 was used to for the data visualization.

Results

The prevalence of drug resistance was found to be: cefixime (CFM) (41.3%), furazolidone (FZD) (34.4%), amoxicillin (AMX) (20.7%), levofloxacin (LVFX) (70.7%), metronidazole (MTZ) (39.6%), tetracycline (TET) (20.7%), clarithromycin (CLA) (17.2%), and rifabutin (RIF) (17.2%). Out of 58 H. pylori strains, 3 were pan susceptible. There were H. pylori strains with single-drug resistance (21.8%, 12/55), dual resistance (30.9%, 17/55), triple resistance (20%, 11/55), and multidrug resistance (27.3%, 15/55). The resistance rate in MTZ, CLA and RIF were found to be significantly higher in females as compared to males (p = 0.005, p = 0.002, and p = 0.02), respectively. The resistance to TET exhibited significantly higher levels in gastritis compared to GERD, DU, and other disease groups (p = 0.04) respectively.

Conclusion

TET, AMX, CLA, and RIF were found to be more effective antibiotics against H. pylori infections, whereas more studies are required to provide evidence on increasing resistance rate of LVFX.

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幽门螺杆菌对八种抗生素的敏感性模式：印度北部的一项研究

背景本分析的目的是评估对幽门螺旋杆菌（H. pylori）菌株有效的八种药物的抗菌敏感性以及幽门螺旋杆菌毒力基因的遗传多样性，以预测北印度的临床结果。材料和方法研究对象包括从各种胃肠道疾病患者体内分离出的 58 株幽门螺杆菌。采用琼脂稀释法测定了各种抗生素的 MICs。采用卡方检验和费舍尔精确检验来确定 p 值，当 p 值≤0.05 时视为显著。数据可视化使用 RStudio 4.0。结果发现耐药率为：头孢克肟（CFM）（41.3%）、呋喃唑酮（FZD）（34.4%）、阿莫西林（AMX）（20.7%）、左氧氟沙星（LVFX）（70.7%）、甲硝唑（MTZ）（39.6%）、四环素（TET）（20.7%）、克拉霉素（CLA）（17.2%）和利福布汀（RIF）（17.2%）。在 58 株幽门螺杆菌中，有 3 株对泛敏感。幽门螺杆菌菌株中存在单耐药（21.8%，12/55）、双耐药（30.9%，17/55）、三耐药（20%，11/55）和多耐药（27.3%，15/55）。与男性相比，女性对 MTZ、CLA 和 RIF 的耐药率明显更高（分别为 p = 0.005、p = 0.002 和 p = 0.02）。与胃食管反流病、DU 和其他疾病组相比，胃炎患者对 TET 的抵抗力明显更高（p = 0.04）。结论 TET、AMX、CLA 和 RIF 被认为是对幽门螺杆菌感染更有效的抗生素，而 LVFX 的耐药率增加还需要更多的研究来证明。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Helicobacter 医学-微生物学

CiteScore

8.40

自引率

9.10%

发文量

审稿时长

2 months

期刊介绍： Helicobacter is edited by Professor David Y Graham. The editorial and peer review process is an independent process. Whenever there is a conflict of interest, the editor and editorial board will declare their interests and affiliations. Helicobacter recognises the critical role that has been established for Helicobacter pylori in peptic ulcer, gastric adenocarcinoma, and primary gastric lymphoma. As new helicobacter species are now regularly being discovered, Helicobacter covers the entire range of helicobacter research, increasing communication among the fields of gastroenterology; microbiology; vaccine development; laboratory animal science.