Single-repeat MYB transcription factor, OsMYB1R, enhanced phytoalexin sakuranetin accumulation and Magnaporthe oryzae resistance

IF 5.4 Q1 PLANT SCIENCES
Yilin Zhang , Jitao Hu , Linying Li , Xueying Zhang , Lili Chen , Zhongjing Zhou , Junmin Wang , Qing Sheng , Zongsuo Liang , Gaojie Hong , Wei Yu , Yuqing He
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Abstract

Sakuranetin, a flavonoid phytoalexin in rice, plays a crucial role in defense against pathogen infection. While MYB-type transcription factors are well-known to regulate plant growth, development, secondary metabolism, and adaptation to environmental stresses, the function of rice MYB-related transcription factors in sakuranetin biosynthesis and sakuranetin-mediated defense remains unclear. In this study, we identified and characterized OsMYB1R, a novel single repeat MYB transcription factor that acts as a transcriptional activator in sakuranetin biosynthesis. Protein-DNA binding and activation assays revealed that OsMYB1R directly regulates the gene promoter of OsNOMT, a key enzyme in sakuranetin synthesis. Molecular analyses and infection studies using OsMYB1R-overexpressing (OsMYB1R-OE) and OsMYB1R-knockout (Osmyb1r, generated using CRISPR/Cas9) plants demonstrated that OsMYB1R increases sakuranetin production and decreases Magnaporthe oryzae infection by transcriptionally regulating OsNOMT expression. This finding indicates a positive regulation of sakuranetin biosynthesis and antifungal resistance by the OsMYB1R-OsNOMT crosstalk. Interestingly, the alteration of OsMYB1R expression did not affect yield-related agronomic traits. Our results reveal a novel and positive role of 1R-MYB in secondary metabolite biosynthesis and pathogen defense, suggesting that OsMYB1R is a potential gene for effectively enhancing rice resistance without compromising yield.

单重复 MYB 转录因子 OsMYB1R 增强了植物雌激素樱草素的积累和木格氏酵母菌的抗性
樱黄素是水稻中的一种黄酮类植物毒素,在抵御病原体感染方面起着至关重要的作用。众所周知,MYB 型转录因子调控植物的生长、发育、次生代谢和对环境胁迫的适应,但水稻 MYB 相关转录因子在樱黄素生物合成和樱黄素介导的防御中的功能仍不清楚。在这项研究中,我们发现并鉴定了一种新型单重复 MYB 转录因子 OsMYB1R,它在樱草素生物合成过程中起着转录激活剂的作用。蛋白-DNA 结合和激活试验显示,OsMYB1R 直接调控樱草素合成过程中的关键酶 OsNOMT 的基因启动子。使用 OsMYB1R 高表达(OsMYB1R-OE)和 OsMYB1R 基因敲除(Osmyb1r,使用 CRISPR/Cas9 生成)植株进行的分子分析和感染研究表明,OsMYB1R 通过转录调控 OsNOMT 的表达,提高了樱草素的产量,并降低了木格氏球菌的感染。这一研究结果表明,OsMYB1R-OsNOMT 的相互作用对樱草素的生物合成和抗真菌性具有正向调节作用。有趣的是,OsMYB1R表达的改变并不影响产量相关的农艺性状。我们的研究结果揭示了 1R-MYB 在次生代谢物生物合成和病原体防御中的一种新的积极作用,表明 OsMYB1R 是一种在不影响产量的情况下有效增强水稻抗性的潜在基因。
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来源期刊
Current Plant Biology
Current Plant Biology Agricultural and Biological Sciences-Plant Science
CiteScore
10.90
自引率
1.90%
发文量
32
审稿时长
50 days
期刊介绍: Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.
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