Prevalence and molecular epidemiology of carbapenemase-producing Enterobacterales isolated from dog and cat faeces submitted to veterinary laboratories in the USA

IF 2.3 2区农林科学 Q3 INFECTIOUS DISEASES

Zoonoses and Public Health Pub Date : 2024-05-15 DOI:10.1111/zph.13144

Jaclyn Dietrich, Tessa E. LeCuyer, G. Kenitra Hendrix, Claire R. Burbick, Megan E. Jacob, Barbara A. Byrne, Karen Olsen, Maria Mitchell, Olgica Ceric, Rachel Lin, Jessica Joneson, Megan Lintner, Alexandra Fox, Diamond McClendon, Trevor Alexander, Kayla Joyce, Melissa Byrd, Jason Clinton, Keith Snipes, Laura Peak, Stephen D. Cole

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引用次数: 0

Abstract

Aims

To estimate the prevalence of carbapenemase-producing Enterobacterales (CPE) carriage among pets using faecal specimens submitted to veterinary diagnostic laboratories throughout the US. A secondary aim was to employ whole-genome sequencing (WGS) to characterize isolates of CPE from companion animals and compare them to publicly available CPE genomes.

Methods and Results

To estimate the prevalence of CPE in companion animals in the USA, a multicenter surveillance study including 8 different veterinary diagnostic laboratories from across the USA was conducted. Briefly, remnant faecal specimens from dogs and cats were screened using two selective agar plates (CHROMID Carba and MacConkey with 1 mg/L cefotaxime and 0.125 mg/L meropenem) and presumptive CPE isolates screened by the modified carbapenemase inactivation method for carbapenemase production. A total of 2393 specimens were screened and yielded 196 isolates for carbapenemase screening. A total of 5 isolates from 4 dogs and 1 cat at 3 different veterinary diagnostic laboratories were confirmed to produce a carbapenemase (0.21%). Whole-genome sequencing (WGS) revealed two E. coli (ST167) isolates that both produced an NDM-5 carbapenemase, two Enterobacter hormaechei (ST171) isolates that produced an NDM-5 carbapenemase and a KPC-4 carbapenemase respectively and one Klebsiella oxytoca (ST199) that produced an Oxa-48-type carbapenemase. Both E. coli isolates were found to be within at least 22 SNPs of previously characterized canine and human CPE isolates.

Conclusions

This study demonstrates that the prevalence of CPE among companion animals is relatively low (0.21%) but that given the genetic relatedness of animal isolates to human isolates, additional surveillance is needed.

Abstract Image

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从提交给美国兽医实验室的狗和猫粪便中分离出的产碳青霉烯酶肠杆菌的流行率和分子流行病学。

目的：利用提交给美国各地兽医诊断实验室的粪便标本，估计宠物体内产碳青霉烯酶肠杆菌（CPE）携带率。另一个目的是利用全基因组测序（WGS）来描述从伴侣动物中分离出的 CPE 的特征，并将它们与公开的 CPE 基因组进行比较：为了估计 CPE 在美国伴侣动物中的流行率，我们开展了一项多中心监测研究，其中包括来自美国各地的 8 个不同的兽医诊断实验室。简而言之，使用两种选择性琼脂平板（CHROMID Carba 和 MacConkey，含 1 毫克/升头孢他啶和 0.125 毫克/升美罗培南）对狗和猫的残余粪便标本进行筛查，并使用改良碳青霉烯酶灭活法筛查碳青霉烯酶的产生情况。共筛查了 2393 份标本，并对其中的 196 份分离物进行了碳青霉烯酶筛查。在 3 个不同的兽医诊断实验室中，共有来自 4 只狗和 1 只猫的 5 个分离物被证实产生了碳青霉烯酶（0.21%）。全基因组测序（WGS）结果显示，两个大肠杆菌（ST167）分离物均产生了NDM-5碳青霉烯酶，两个荷马肠杆菌（ST171）分离物分别产生了NDM-5碳青霉烯酶和KPC-4碳青霉烯酶，一个克雷伯菌（ST199）产生了Oxa-48型碳青霉烯酶。发现这两种大肠杆菌分离物与之前鉴定的犬和人类 CPE 分离物至少存在 22 个 SNPs：这项研究表明，伴侣动物 CPE 的发病率相对较低（0.21%），但鉴于动物分离物与人类分离物在基因上的相关性，需要进行更多的监测。

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来源期刊

Zoonoses and Public Health 医学-传染病学

CiteScore

5.30

自引率

4.20%

发文量

115

审稿时长

6-12 weeks

期刊介绍： Zoonoses and Public Health brings together veterinary and human health researchers and policy-makers by providing a venue for publishing integrated and global approaches to zoonoses and public health. The Editors will consider papers that focus on timely collaborative and multi-disciplinary research in zoonoses and public health. This journal provides rapid publication of original papers, reviews, and potential discussion papers embracing this collaborative spirit. Papers should advance the scientific knowledge of the sources, transmission, prevention and control of zoonoses and be authored by scientists with expertise in areas such as microbiology, virology, parasitology and epidemiology. Articles that incorporate recent data into new methods, applications, or approaches (e.g. statistical modeling) which enhance public health are strongly encouraged.