SoxB family genes delay regeneration and cause abnormal movement in Dugesia japonica.

IF 2.3 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yibo Yang, Ziyi Lin, Nannan Li, Ning Li, Dezeng Liu, Zimei Dong, Guangwen Chen
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Abstract

SoxB subfamily is an important branch of Sox family and plays a key role in animal physiological process, but little is known about their function in planarian regeneration. This study aims to evaluate the function of DjSoxB family genes in intact and regenerating planarians Dugesia japonica. Here, we amplify the full-length cDNA of DjSoxB1 and DjSoxB2 in D. japonica by rapid amplification of the cDNA ends (RACE), detect the expression of DjSoxB family genes in planarian. The results show that DjSoxBs are expressed in parenchymal tissue and the hybridization signals partially disappear after irradiation indicates DjSoxB family genes are expressed in neoblasts. After the RNA interference (RNAi) of DjSoxB1, DjSoxB2 and DjSoxB3 separately, the numbers of proliferative cells are all reduced that causes planarians show slower growth of blastema in the early stage of regeneration, and nerves of planarians are affected that the movement speed of planarians decreases in varying degrees. Specially, planarians in the DjSoxB3 RNAi group show shrinkage and twisting. Overall, this study reveals that DjSoxB family genes play a role in cell proliferation during regeneration. They also play an important role in the maintenance of normal nerve function and nerve regeneration. These results provide directions for the functional study of SoxB family genes and provide an important foundation for planarian regeneration.

Abstract Image

SoxB 家族基因会延迟再生,并导致日本杉属植物的异常运动。
SoxB 亚家族是 Sox 家族的一个重要分支,在动物生理过程中起着关键作用,但人们对其在扁形动物再生过程中的功能知之甚少。本研究旨在评估DjSoxB家族基因在完整和再生刨花中的功能。本文通过快速扩增 cDNA末端(RACE)技术扩增了DjSoxB1和DjSoxB2的全长cDNA,并检测了DjSoxB家族基因在刨食者中的表达。结果表明,DjSoxBs在实质组织中表达,照射后杂交信号部分消失,表明DjSoxB家族基因在新生细胞中表达。分别对DjSoxB1、DjSoxB2和DjSoxB3进行RNA干扰(RNAi)后,增殖细胞的数量均减少,导致刨花在再生早期的胚泡生长缓慢,刨花的神经受到影响,刨花的运动速度有不同程度的下降。特别是,DjSoxB3 RNAi 组的刨花出现萎缩和扭曲。总之,这项研究揭示了DjSoxB家族基因在再生过程中对细胞增殖的作用。它们在维持正常神经功能和神经再生方面也发挥着重要作用。这些研究结果为SoxB家族基因的功能研究提供了方向,并为平面动物的再生提供了重要的基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Molecular Genetics and Genomics
Molecular Genetics and Genomics 生物-生化与分子生物学
CiteScore
5.10
自引率
3.20%
发文量
134
审稿时长
1 months
期刊介绍: Molecular Genetics and Genomics (MGG) publishes peer-reviewed articles covering all areas of genetics and genomics. Any approach to the study of genes and genomes is considered, be it experimental, theoretical or synthetic. MGG publishes research on all organisms that is of broad interest to those working in the fields of genetics, genomics, biology, medicine and biotechnology. The journal investigates a broad range of topics, including these from recent issues: mechanisms for extending longevity in a variety of organisms; screening of yeast metal homeostasis genes involved in mitochondrial functions; molecular mapping of cultivar-specific avirulence genes in the rice blast fungus and more.
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