T6SS and T4SS Redundantly Secrete Effectors to Govern the Virulence and Bacterial Competition in Pectobacterium PccS1.

IF 2.6 2区农林科学 Q2 PLANT SCIENCES

Phytopathology Pub Date : 2024-08-01 Epub Date: 2024-08-05 DOI:10.1094/PHYTO-11-23-0455-R

Shu Che, Chen Sun, Liuke Yang, Min Zhou, Lingyan Xia, Jingyuan Yan, Mengyi Jiang, Jiaju Wang, Huan Wang, Wenjun Zhao, Ian Toth, Baishi Hu, Tao Guo, Jiaqin Fan

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引用次数: 0

Abstract

Previous studies revealed that the type VI secretion system (T6SS) has an essential role in bacterial competition and virulence in many gram-negative bacteria. However, the role of T6SS in virulence in Pectobacterium atrosepticum remains controversial. We examined a closely related strain, PccS1, and discovered that its T6SS comprises a single-copy cluster of 17 core genes with a higher identity to homologs from P. atrosepticum. Through extensive phenotypic and functional analyses of over 220 derivatives of PccS1, we found that three of the five VgrGs could be classified into group I VgrGs. These VgrGs interacted with corresponding DUF4123 domain proteins, which were secreted outside of the membrane and were dependent on either the T6SS or type IV secretion system (T4SS). This interaction directly governed virulence and competition. Meanwhile, supernatant proteomic analyses with strains defective in the T6SS and/or T4SS confirmed that effectors, such as FhaB, were secreted redundantly to control the virulence and suppress host callose deposition in the course of infection. Notably, this redundant secretion mechanism between the T6SS and T4SS is believed to be the first of its kind in bacteria.

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T6SS 和 T4SS 冗余分泌效应器，控制果胶杆菌 PccS1 的毒力和细菌竞争。

以往的研究表明，Ⅵ型分泌系统（T6SS）在许多革兰氏阴性细菌的细菌竞争和毒力中发挥着重要作用。然而，T6SS 在无核果胶杆菌毒力中的作用仍存在争议。我们研究了一个密切相关的菌株 PccS1，发现它的 T6SS 包括一个由 17 个核心基因组成的单拷贝基因簇，与无核果胶杆菌的同源基因有更高的同一性。通过对 PccS1 的 220 多个衍生物进行广泛的表型和功能分析，我们发现五个 VgrGs 中的三个可归类为第一组 VgrGs。这些VgrGs与相应的DUF4123结构域蛋白相互作用，这些蛋白在膜外分泌，依赖于T6SS或T4SS。这种相互作用直接决定了病毒的毒性和竞争性。同时，对 T6SS 或/和 T4SS 缺陷染色的上清液蛋白质组分析证实，FhaB 等效应物在感染过程中通过冗余分泌来控制毒力和抑制宿主胼胝质沉积。值得注意的是，T6SS 和 T4SS 之间的这种冗余分泌机制被认为是细菌中的首例。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Phytopathology 生物-植物科学

CiteScore

5.90

自引率

9.40%

发文量

505

审稿时长

4-8 weeks

期刊介绍： Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.