Defects in integrin complex formation promote CHKB-mediated muscular dystrophy.

IF 3.3 2区 生物学 Q1 BIOLOGY
Life Science Alliance Pub Date : 2024-05-15 Print Date: 2024-08-01 DOI:10.26508/lsa.202301956
Mahtab Tavasoli, Christopher R McMaster
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引用次数: 0

Abstract

Phosphatidylcholine (PC) is the major membrane phospholipid in most eukaryotic cells. Bi-allelic loss of function variants in CHKB, encoding the first step in the synthesis of PC, is the cause of a rostrocaudal muscular dystrophy in both humans and mice. Loss of sarcolemma integrity is a hallmark of muscular dystrophies; however, how this occurs in the absence of choline kinase function is not known. We determine that in Chkb -/- mice there is a failure of the α7β1 integrin complex that is specific to affected muscle. We observed that in Chkb -/- hindlimb muscles there is a decrease in sarcolemma association/abundance of the PI(4,5)P2 binding integrin complex proteins vinculin, and α-actinin, and a decrease in actin association with the sarcolemma. In cells, pharmacological inhibition of choline kinase activity results in internalization of a fluorescent PI(4,5)P2 reporter from discrete plasma membrane clusters at the cell surface membrane to cytosol, this corresponds with a decreased vinculin localization at plasma membrane focal adhesions that was rescued by overexpression of CHKB.

整合素复合物形成缺陷会促进 CHKB 介导的肌肉萎缩症。
磷脂酰胆碱(PC)是大多数真核细胞的主要膜磷脂。编码 PC 合成第一步的 CHKB 双等位基因功能缺失变体是人类和小鼠喙尾肌营养不良症的病因。肌浆蛋白完整性的丧失是肌肉萎缩症的一个特征;然而,在缺乏胆碱酯酶功能的情况下,这种丧失是如何发生的尚不清楚。我们确定,在 Chkb -/- 小鼠中,α7β1 整合素复合物失效,这是受影响肌肉的特异性。我们观察到,在 Chkb -/-后肢肌肉中,肌浆膜与 PI(4,5)P2 结合的整合素复合物蛋白 vinculin 和 α-肌动蛋白的结合/丰度降低,肌动蛋白与肌浆膜的结合减少。在细胞中,对胆碱酯酶活性的药理抑制会导致荧光 PI(4,5)P2 报告物从细胞表面膜的离散质膜簇内化到细胞膜,这与质膜焦点粘附处 vinculin 定位减少相对应,而过量表达 CHKB 可挽救这种情况。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Life Science Alliance
Life Science Alliance Agricultural and Biological Sciences-Plant Science
CiteScore
5.80
自引率
2.30%
发文量
241
审稿时长
10 weeks
期刊介绍: Life Science Alliance is a global, open-access, editorially independent, and peer-reviewed journal launched by an alliance of EMBO Press, Rockefeller University Press, and Cold Spring Harbor Laboratory Press. Life Science Alliance is committed to rapid, fair, and transparent publication of valuable research from across all areas in the life sciences.
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