Anti-cytokine Storm Activity of Fraxin, Quercetin, and their Combination on Lipopolysaccharide-Induced Cytokine Storm in Mice: Implications in COVID-19.

IF 1.6 Q2 MEDICINE, GENERAL & INTERNAL
Nada Sahib Shaker, Hayder B Sahib, Nibras J Tahseen
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引用次数: 0

Abstract

Background: Cytokine release syndrome (CRS) is the leading cause of mortality in advanced stages of coronavirus patients. This study examined the prophylactic effects of fraxin, quercetin, and a combination of fraxin+quercetin (FQ) on lipopolysaccharide-induced mice.

Methods: Sixty mice were divided into six groups (n=10) as follows: control, LPS only, fraxin (120 mg/Kg), quercetin (100 mg/Kg), dexamethasone (5 mg/Kg), and FQ. All treatments were administered intraperitoneally (IP) one hour before induction by LPS (5 mg/Kg) IP injection. Twenty-four hours later, the mice were euthanized. Interleukin one beta (IL-1β), interleukin 6 (IL-6), and tumor necrosis factor-alpha (TNF-α) were quantified using an enzyme-linked immunosorbent assay (ELISA), and lung and kidney tissues were examined for histopathological alterations. This study was conducted at Al-Nahrain University, Baghdad, Iraq, in 2022.

Results: FQ reduced IL-1β (P<0.001). All treatments significantly suppressed IL-6, fraxin, quercetin, dexamethasone, and FQ, all with P<0.001. The TNF-α level was reduced more with dexamethasone (P<0.001) and quercetin (P<0.001). Histopathological scores were significantly reduced mainly by quercetin and FQ in the lungs with scores of 12.30±0.20 (P=0.093), and 15.70±0.20 (P=0.531), respectively. The scores were 13±0.26 (P=0.074) and 15±0.26 (P=0.222) for quercetin and FQ in the kidneys, respectively.

Conclusion: All used treatments reduced proinflammatory cytokine levels and protected against LPS-induced tissue damage.

梣酮、槲皮素及其复方制剂对脂多糖诱导的小鼠细胞因子风暴的抗细胞因子风暴活性:COVID-19 的意义
背景:细胞因子释放综合征(CRS细胞因子释放综合征(CRS)是冠状病毒晚期患者死亡的主要原因。本研究探讨了枸杞黄素、槲皮素和枸杞黄素+槲皮素组合(FQ)对脂多糖诱导的小鼠的预防作用:将60只小鼠分为以下6组(n=10):对照组、仅LPS组、氟辛(120毫克/千克)组、槲皮素(100毫克/千克)组、地塞米松(5毫克/千克)组和FQ组。所有治疗均在小鼠腹腔注射 LPS(5 毫克/千克)诱导前一小时进行。24 小时后,小鼠被安乐死。使用酶联免疫吸附试验(ELISA)对白细胞介素一β(IL-1β)、白细胞介素六(IL-6)和肿瘤坏死因子-α(TNF-α)进行定量分析,并检查肺和肾组织的组织病理学改变。这项研究于 2022 年在伊拉克巴格达 Al-Nahrain 大学进行:结果:FQ 降低了 IL-1β (PC结论:所有使用的治疗方法都降低了促炎症反应:结果:FQ 降低了 IL-1β (PConclusion),所有使用的治疗方法都降低了促炎细胞因子水平,防止了 LPS 诱导的组织损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Iranian Journal of Medical Sciences
Iranian Journal of Medical Sciences MEDICINE, GENERAL & INTERNAL-
CiteScore
3.20
自引率
0.00%
发文量
84
审稿时长
12 weeks
期刊介绍: The Iranian Journal of Medical Sciences (IJMS) is an international quarterly biomedical publication, which is sponsored by Shiraz University of Medical Sciences. The IJMS intends to provide a scientific medium of com­muni­cation for researchers throughout the globe. The journal welcomes original clinical articles as well as clinically oriented basic science re­search experiences on prevalent diseases in the region and analysis of various regional problems.
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