Immunochromatography Lateral Flow Strip Enhancement Based on Passive Gold Nanoparticles Conjugation to Detect Schistosma haematobium Antigens in Human Serum

IF 1.2 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica Pub Date : 2024-05-16 DOI:10.1007/s11686-024-00841-y

Mahmoud N. El-Shall, Ibrahim Aly, Alaa Samen, Wesam M. Salama, Fadi Baakdah

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引用次数: 0

Abstract

Purpose

This study aimed to develop and evaluate a lateral flow card for the detection of active Schistosoma haematobium infection.

Methods

In order to prepare the immunochromatography lateral flow strip (ICLFS), antibodies purified from schistosomiasis were conjugated passively with gold nanoparticles using a potassium carbonate buffer.

Results

The novel ICLFS was able to correctly identify 64 out of 67 samples of schistosomiasis, 6 out of 90 samples of other parasites, and 0 out of 27 control samples. Sensitivity, specificity, negative predictive value (NPV), and positive predictive value (PPV) were 95.5%, 93.3%, 90%, and 91.4% respectively. Comparatively, the sensitivity, specificity, NPV, and PPV of sandwich enzyme-linked immunosorbent assays (ELISA) conjugated with gold nanoparticles (AuNPs) were 91.1%, 88.8%, 85.9%, and 84.4% respectively. The increased sensitivity and specificity of ICLFS produced superior results to those of sandwich ELISA.

Conclusion

In conclusion, ICLFS is more beneficial and precise than sandwich ELISA for detection of S. haematobium infection at early stage.

Abstract Image

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基于被动金纳米粒子共轭的免疫层析侧流条带增强技术检测人血清中的血吸虫抗原

目的：本研究旨在开发和评估一种用于检测血吸虫活动感染的侧流显像卡：为了制备免疫层析侧流条（ICLFS），使用碳酸钾缓冲液将从血吸虫中纯化的抗体与金纳米颗粒被动共轭：结果：新型 ICLFS 能正确识别 67 份血吸虫样本中的 64 份、90 份其他寄生虫样本中的 6 份和 27 份对照样本中的 0 份。灵敏度、特异性、阴性预测值（NPV）和阳性预测值（PPV）分别为 95.5%、93.3%、90% 和 91.4%。相比之下，与金纳米粒子（AuNPs）结合的夹心酶联免疫吸附试验（ELISA）的灵敏度、特异性、NPV 和 PPV 分别为 91.1%、88.8%、85.9% 和 84.4%。ICLFS 灵敏度和特异性的提高使其结果优于夹心酶联免疫吸附试验：总之，ICLFS 在早期检测血吸虫感染方面比夹心酶联免疫吸附法更有效、更精确。

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来源期刊

Acta Parasitologica 医学-寄生虫学

CiteScore

3.10

自引率

6.70%

发文量

149

审稿时长

6-12 weeks

期刊介绍： Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.