Growth dynamics and lipid metabolism of Aurantiochytrium sp.: insights into its potential applications

IF 1.7 4区环境科学与生态学 Q3 ECOLOGY

Aquatic Ecology Pub Date : 2024-05-11 DOI:10.1007/s10452-024-10105-6

Zahra Shafaghat, Farzaneh Najafi, Ramazan-Ali Khavari-Nejad, Mehdi Mohammadi, Sattar Tahmasebi Enferadi

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Abstract

Aurantiochytrium sp. is a marine microalga known for its bioactive compounds. This study assessed its presence in Iran and investigated its specific biochemical and molecular traits. Parameters such as biomass, protein content, hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) levels, catalase enzyme (CAT) activity, and expression of malic enzyme (ME) and acetyl-CoA carboxylase (ACCase) genes were analyzed at different growth stages (24, 48, 72, and 96 h). Fatty acid composition, unsaturated fatty acids (UFA) and saturated fatty acids (SFA) contents, and the ratio of UFA to SFA were also examined. Molecular identification confirmed successful proliferation, with significant increases in biomass and protein content during growth. Elevated H₂O₂ and MDA levels indicated oxidative stress, whereas enhanced CAT activity suggested its potential role in scavenging reactive oxygen species and mitigating oxidative damage. The upregulation of ME and ACCase genes during growth indicated their involvement in lipid biosynthesis. This was further supported by the analysis of fatty acid composition, which revealed a higher proportion of UFA than SFA. These findings underscore the importance of specific growth stages for optimizing cultivation conditions and maximizing bioactive compound production in Aurantiochytrium sp. Overall, this study provides valuable insights into the growth dynamics and lipid metabolism of Aurantiochytrium sp., contributing to our understanding of its potential applications.

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Aurantiochytrium sp.的生长动力学和脂质代谢：对其潜在应用的启示

Aurantiochytrium sp. 是一种海洋微藻，以其生物活性化合物而闻名。本研究评估了其在伊朗的存在，并调查了其特定的生化和分子特征。在不同生长阶段（24、48、72 和 96 小时）分析了生物量、蛋白质含量、过氧化氢（H2O2）和丙二醛（MDA）水平、过氧化氢酶（CAT）活性、苹果酸酶（ME）和乙酰-CoA 羧化酶（ACCase）基因的表达等参数。还检测了脂肪酸组成、不饱和脂肪酸（UFA）和饱和脂肪酸（SFA）含量以及 UFA 与 SFA 的比例。分子鉴定证实了增殖成功，生长过程中生物量和蛋白质含量显著增加。H2O2 和 MDA 水平的升高表明存在氧化应激，而 CAT 活性的增强表明其在清除活性氧和减轻氧化损伤方面发挥了潜在作用。生长过程中 ME 和 ACCase 基因的上调表明它们参与了脂质的生物合成。脂肪酸组成分析进一步证实了这一点，该分析表明 UFA 的比例高于 SFA。这些发现强调了特定生长阶段对优化 Aurantiochytrium sp.的培养条件和最大限度地提高生物活性化合物产量的重要性。总体而言，本研究为了解 Aurantiochytrium sp.的生长动态和脂质代谢提供了宝贵的见解，有助于我们了解其潜在的应用领域。

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来源期刊

Aquatic Ecology 环境科学-海洋与淡水生物学

CiteScore

3.90

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Aquatic Ecology publishes timely, peer-reviewed original papers relating to the ecology of fresh, brackish, estuarine and marine environments. Papers on fundamental and applied novel research in both the field and the laboratory, including descriptive or experimental studies, will be included in the journal. Preference will be given to studies that address timely and current topics and are integrative and critical in approach. We discourage papers that describe presence and abundance of aquatic biota in local habitats as well as papers that are pure systematic. The journal provides a forum for the aquatic ecologist - limnologist and oceanologist alike- to discuss ecological issues related to processes and structures at different integration levels from individuals to populations, to communities and entire ecosystems.