A medium‐throughput approach for improved taxonomic identification of lipids preserved in ancient pottery

IF 1.5 3区地球科学 0 ARCHAEOLOGY

Archaeometry Pub Date : 2024-05-07 DOI:10.1111/arcm.12976

Léa Drieu, Jasmine Lundy, Rachel K. Smith, Ed Bergström, Helen Talbot, Milena Primavera, Girolamo Fiorentino, Oliver E. Craig, Jane Thomas‐Oates

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Abstract

Organic residue analysis (ORA) is a valuable tool for the study of ancient diets, but conventional methods remain limited in terms of taxonomic identification or to resolve mixtures. Here, we propose a method to further explore a class of compounds—triacylglycerols (TAGs)—using high‐resolution mass spectrometry to overcome these limitations in an attempt to better characterise culinary practices. Over 70 medieval Sicilian pots and a wide range of authentic fresh products were studied by matrix‐assisted laser desorption/ionisation–mass spectrometry (MALDI‐MS and MALDI‐MS/MS). MALDI‐MS analysis can distinguish fresh foodstuffs but provides little additional information regarding the contents of archaeological pottery compared to conventional ORA methods. In contrast, product ion analyses were able to deconvolute a range of animal carcass fat mixtures. In addition, detailed analysis of the composition of saturated T44 and unsaturated T50–T54 TAGs was able to provide greater taxonomic resolution regarding dairy products and plant oils.

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改进古陶器中保存的脂质分类鉴定的中等通量方法

有机残留物分析（ORA）是研究古代饮食的重要工具，但传统方法在分类鉴定或解决混合物方面仍然存在局限性。在此，我们提出了一种利用高分辨率质谱进一步探索三酰甘油（TAGs）这类化合物的方法，以克服这些局限性，尝试更好地描述烹饪方法。研究人员利用基质辅助激光解吸/电离质谱（MALDI-MS 和 MALDI-MS/MS）对 70 多种中世纪西西里锅和各种地道的新鲜产品进行了研究。与传统的 ORA 方法相比，MALDI-MS 分析可以区分新鲜食品，但对考古陶器的内容提供的额外信息很少。与此相反，产物离子分析能够解构一系列动物尸体脂肪混合物。此外，对饱和 T44 和不饱和 T50-T54 TAGs 成分的详细分析能够提供有关乳制品和植物油的更高分类分辨率。

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来源期刊

Archaeometry 地学-地球科学综合

CiteScore

3.60

自引率

12.50%

发文量

105

审稿时长

6 months

期刊介绍： Archaeometry is an international research journal covering the application of the physical and biological sciences to archaeology, anthropology and art history. Topics covered include dating methods, artifact studies, mathematical methods, remote sensing techniques, conservation science, environmental reconstruction, biological anthropology and archaeological theory. Papers are expected to have a clear archaeological, anthropological or art historical context, be of the highest scientific standards, and to present data of international relevance. The journal is published on behalf of the Research Laboratory for Archaeology and the History of Art, Oxford University, in association with Gesellschaft für Naturwissenschaftliche Archäologie, ARCHAEOMETRIE, the Society for Archaeological Sciences (SAS), and Associazione Italian di Archeometria.