Electrochemical immunomagnetic assay for interleukin-6 detection in human plasma

IF 3.5 Q2 CHEMISTRY, ANALYTICAL
Grace Buckey, Olivia E. Owens, Hannah A. Richards and David E. Cliffel
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引用次数: 0

Abstract

An electrochemical immunoassay for interleukin-6 (IL-6) was developed based on IL-6 capture using magnetic beads and electrochemical signal production using horseradish peroxidase/tetramethylbenzidine. We achieved IL-6 detection from the 50–1000 pg mL−1 range, which is a physiologically relevant IL-6 range for a variety of biological systems. The sandwich assay performed well in phosphate buffered solution as well as in cellular media and human plasma spiked with IL-6, and decreased time to IL-6 concentration readout to approximately one hour. There is also future potential to apply this assay to real-time point-of-care human disease diagnostics.

Abstract Image

Abstract Image

用于检测人体血浆中白细胞介素-6 的电化学免疫磁分析法
基于使用磁珠捕获 IL-6 和使用辣根过氧化物酶/四甲基联苯胺产生电化学信号的方法,我们开发了一种白细胞介素-6(IL-6)电化学免疫测定。我们实现了 50-1000 pg mL-1 范围内的 IL-6 检测,这是多种生物系统中与生理相关的 IL-6 检测范围。夹心检测法在磷酸盐缓冲溶液、细胞介质和加有 IL-6 的人体血浆中均表现良好,而且读出 IL-6 浓度的时间缩短到约一小时。未来,这种测定法还有可能应用于人类疾病的实时护理点诊断。
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CiteScore
2.30
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0.00%
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