Design and proof-of-concept of a micropillar-based microfluidic chip for trapping and culture of single cells

IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION
Thu Hang Nguyen, Ngoc Anh Nguyen Thi, Hang Bui Thu, Tung Thanh Bui, Trinh Chu Duc, Loc Do Quang
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Abstract

Single-cell analysis provides a groundbreaking avenue for exploring cell-to-cell variation, the heterogeneity of cell responses to stimuli, and the impact of DNA sequence variations on cell phenotypes. A crucial facet of this analytical approach involves the refinement of techniques for effective single-cell trapping and sustained culture. This study introduces a microfluidic platform based on micropillars for hydrodynamic trapping and prolonged cultivation of individual cells. The proposed biochip design, termed three-micropillars based microfluidic (3µPF) structure, incorporates interleaved trap units, each featuring three-micropillars based microfluidic structure strategically designated to trap single cells, enhance the surface area of cells exposed to the culture medium, and enable dynamic culture, continuous waste removal. This configuration aims to mitigate adverse effects associated with bioparticle collisions compared to conventional trap units. The study employs finite element method to conduct a comprehensive numerical investigation into the operational mechanism of the microfluidic device. The simulation results show that the filled trap unit demonstrates a low-velocity magnitude, reducing shear stress on cells and facilitating extended culture. The hydrodynamic single-cell trap mechanism of the proposed device was also verified. The insights derived from this work are pivotal for optimizing the device and guiding future experimental examinations, thus contributing significantly to the progression of single-cell analysis techniques.

Abstract Image

设计并验证基于微柱的微流体芯片,用于捕获和培养单细胞
单细胞分析为探索细胞间变异、细胞对刺激反应的异质性以及 DNA 序列变异对细胞表型的影响提供了一条突破性途径。这种分析方法的一个重要方面是完善有效的单细胞捕获和持续培养技术。本研究介绍了一种基于微柱的微流体平台,用于单个细胞的流体动力捕获和长时间培养。所提出的生物芯片设计被称为基于三微柱的微流体(3µPF)结构,它包含交错的捕获单元,每个单元都有三个基于微柱的微流体结构,这些微流体结构被战略性地指定用于捕获单个细胞,增加细胞暴露于培养基的表面积,并实现动态培养和持续清除废物。与传统的捕集装置相比,这种配置旨在减轻生物颗粒碰撞带来的不利影响。研究采用有限元法对微流体装置的运行机制进行了全面的数值研究。模拟结果表明,填充式捕集装置具有低速幅度,可减少细胞受到的剪切应力,有利于延长培养时间。此外,还验证了拟议装置的流体力学单细胞捕获机制。这项工作得出的见解对于优化该装置和指导未来的实验研究至关重要,从而为单细胞分析技术的发展做出了重大贡献。
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来源期刊
Microfluidics and Nanofluidics
Microfluidics and Nanofluidics 工程技术-纳米科技
CiteScore
4.80
自引率
3.60%
发文量
97
审稿时长
2 months
期刊介绍: Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include: 1.000 Fundamental principles of micro- and nanoscale phenomena like, flow, mass transport and reactions 3.000 Theoretical models and numerical simulation with experimental and/or analytical proof 4.000 Novel measurement & characterization technologies 5.000 Devices (actuators and sensors) 6.000 New unit-operations for dedicated microfluidic platforms 7.000 Lab-on-a-Chip applications 8.000 Microfabrication technologies and materials Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).
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