Rhizobacterial community and growth-promotion trait characteristics of Zea mays L. inoculated with Pseudomonas fluorescens UM270 in three different soils.

IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Folia microbiologica Pub Date : 2024-12-01 Epub Date: 2024-05-15 DOI:10.1007/s12223-024-01171-2
Gustavo Santoyo, Carlos Urtis-Flores, Ma Del Carmen Orozco-Mosqueda
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引用次数: 0

Abstract

There is an increasing demand for bioinoculants based on plant growth-promoting rhizobacteria (PGPR) for use in agricultural ecosystems. However, there are still concerns and limited data on their reproducibility in different soil types and their effects on endemic rhizosphere communities. Therefore, this study explored the effects of inoculating the PGPR, Pseudomonas fluorescens strain UM270, on maize growth (Zea mays L.) and its associated rhizosphere bacteriome by sequencing the 16S ribosomal genes under greenhouse conditions. The results showed that inoculation with PGPR P. fluorescens UM270 improved shoot and root dry weights, chlorophyll concentration, and total biomass in the three soil types evaluated (clay, sandy-loam, and loam) compared to those of the controls. Bacterial community analysis of the three soil types revealed that maize plants inoculated with the UM270 strain showed a significant increase in Proteobacteria and Acidobacteria populations, whereas Actinobacteria and Bacteroidetes decreased. Shannon, Pielou, and Faith alpha-biodiversity indices did not reveal significant differences between treatments. Beta diversity revealed a bacterial community differential structure in each soil type, with some variation among treatments. Finally, some bacterial groups were found to co-occur and co-exclude with respect to UM270 inoculation. Considered together, these results show that PGPR P. fluorescens UM270 increases maize plant growth and has an important effect on the resident rhizobacterial communities of each soil type, making it a potential agricultural biofertilizer.

Abstract Image

在三种不同土壤中接种荧光假单胞菌 UM270 的玉米根瘤菌群落和生长促进性状特征。
在农业生态系统中使用基于植物生长促进根瘤菌(PGPR)的生物絮凝剂的需求越来越大。然而,人们对其在不同土壤类型中的可重复性及其对当地根瘤菌群落的影响仍心存疑虑,相关数据也十分有限。因此,本研究通过对温室条件下的 16S 核糖体基因进行测序,探讨了接种 PGPR(荧光假单胞菌 UM270 菌株)对玉米(Zea mays L.)生长及其相关根瘤菌群的影响。结果表明,与对照组相比,接种 PGPR P. fluorescens UM270 能提高所评价的三种土壤(粘土、沙壤土和壤土)中嫩枝和根的干重、叶绿素浓度和总生物量。对三种土壤类型进行的细菌群落分析表明,接种了 UM270 菌株的玉米植株中,蛋白质细菌和酸性细菌的数量显著增加,而放线菌和类杆菌则有所减少。香农(Shannon)、皮鲁(Pielou)和费斯(Faith)α-生物多样性指数未显示出不同处理之间的显著差异。Beta 多样性显示了每种土壤类型中细菌群落的差异结构,不同处理之间存在一些差异。最后,在 UM270 接种的情况下,发现一些细菌群共同出现或共同消失。综合来看,这些结果表明 PGPR P. fluorescens UM270 可以提高玉米植株的生长,并对每种土壤类型中的常驻根瘤菌群落产生重要影响,使其成为一种潜在的农业生物肥料。
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来源期刊
Folia microbiologica
Folia microbiologica 工程技术-生物工程与应用微生物
CiteScore
5.80
自引率
0.00%
发文量
82
审稿时长
6-12 weeks
期刊介绍: Unlike journals which specialize ever more narrowly, Folia Microbiologica (FM) takes an open approach that spans general, soil, medical and industrial microbiology, plus some branches of immunology. This English-language journal publishes original papers, reviews and mini-reviews, short communications and book reviews. The coverage includes cutting-edge methods and promising new topics, as well as studies using established methods that exhibit promise in practical applications such as medicine, animal husbandry and more. The coverage of FM is expanding beyond Central and Eastern Europe, with a growing proportion of its contents contributed by international authors.
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