Strategies for efficient enrichment of anaerobic ammonia oxidizing bacteria in activated sludge

IF 5.9 2区 环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES
Bin Tang , Jin Wang , Xingdong Gao , Zhihua Li
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Abstract

Anaerobic ammonia oxidation (Anammox) is an economical and sustainable wastewater nitrogen removal technology, and its application in the mainstream process is the inevitable trend of the development of Anammox. However, how to effectively enriching Anammox bacteria from the activated sludge remains challenging and restricts its extensive applications. In this study, the rapid and efficient enrichment of Anammox bacteria was achieved by raising the reflux ratio and nitrogen loading rate (NLR) using conventional activated sludge as the inoculant. In the screening phase (days 1–90), the reflux ratio was increased to discharge partial floc sludge, resulting in the relative abundance of Candidatus Brocadiaceae increased from 0.04% to 22.54%, which effectively reduced the matrix and spatial competition between other microorganisms and Anammox bacteria. On day 90, the stoichiometric ratio of the Anammox process closely approached the theoretical value of 1:1.32:0.26, indicating that the Anammox reaction was the primary nitrogen removal process in the system. In the enrichment phase (days 91–238), the NLR increased from 0.43 to 1.20 kgN/(m3·d) and removal efficiency was 71.89%, resulting in the relative abundance of Candidatus Brocadiaceae increased to 61.27% on day 180. The reactor operated steadily from days 444 to 498, maintaining the nitrogen removal rate (NRR) of 3.00 kgN/(m3·d) and achieving successful sludge granulation with the particle size of 392.4 µm. In short, this study provided a simple and efficient approach for enriching Anammox bacteria from the activated sludge, supporting to start an Anammox process efficiently.

Abstract Image

活性污泥中厌氧氨氧化菌的高效增殖策略
厌氧氨氧化(Anammox)是一种经济、可持续的污水脱氮技术,将其应用于主流工艺是厌氧氨氧化发展的必然趋势。然而,如何有效地从活性污泥中富集 Anammox 细菌仍是一项挑战,限制了其广泛应用。本研究以传统活性污泥为接种剂,通过提高回流比和氮负荷率(NLR)实现了 Anammox 细菌的快速高效富集。在筛选阶段(第 1-90 天),通过提高回流比来排放部分絮状污泥,从而使 Candidatus Brocadiaceae 的相对丰度从 0.04% 提高到 22.54%,有效减少了其他微生物与 Anammox 菌之间的基质和空间竞争。第 90 天,Anammox 过程的化学计量比接近理论值 1:1.32:0.26,表明 Anammox 反应是系统中主要的脱氮过程。在富集阶段(第 91-238 天),NLR 从 0.43 kgN/(m3-d) 增加到 1.20 kgN/(m3-d),去除效率为 71.89%,因此在第 180 天,Candidatus Brocadiaceae 的相对丰度增加到 61.27%。反应器在第 444 天至第 498 天稳定运行,氮去除率(NRR)保持在 3.00 kgN/(m3-d),并成功实现了污泥颗粒化,颗粒大小为 392.4 µm。总之,这项研究为从活性污泥中富集 Anammox 细菌提供了一种简单而有效的方法,为高效启动 Anammox 工艺提供了支持。
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来源期刊
Journal of Environmental Sciences-china
Journal of Environmental Sciences-china 环境科学-环境科学
CiteScore
13.70
自引率
0.00%
发文量
6354
审稿时长
2.6 months
期刊介绍: The Journal of Environmental Sciences is an international journal started in 1989. The journal is devoted to publish original, peer-reviewed research papers on main aspects of environmental sciences, such as environmental chemistry, environmental biology, ecology, geosciences and environmental physics. Appropriate subjects include basic and applied research on atmospheric, terrestrial and aquatic environments, pollution control and abatement technology, conservation of natural resources, environmental health and toxicology. Announcements of international environmental science meetings and other recent information are also included.
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