Orbea variegata (L.) Haw in skin carcinogenesis: insights from an in vivo male Swiss mouse model study.

IF 2.3 4区 医学 Q3 ENVIRONMENTAL SCIENCES
Oumaima Chgari, Hicham Wahnou, Martin Ndayambaje, Fatimazahra Moukhfi, Ouafae Benkhnigue, Farida Marnissi, Youness Limami, Mounia Oudghiri
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引用次数: 0

Abstract

Skin cancer is the most widespread type of malignant tumor representing a major public health concern. Considering the numerous side effects associated with conventional treatments, phytotherapy may be regarded as a viable medicinal alternative. This study aimed to investigate the therapeutic potential of Orbea variegata (L.) Haw, an ornamental plant, in treating skin cancer using an animal model induced by a combination of ultraviolet (UV) irradiation and sulfuric acid treatment. The hydroethanolic extract of Orbea variegata underwent phytochemical characterization, identifying the presence of reducing sugars, coumarins, alkaloids, flavonoids, tannins, and saponins through qualitative screening. Quantitative analysis demonstrated significant amounts of phenolic compounds (29.435 ± 0.571 mg GAE/g of dry extract), flavonoids (6.711 ± 0.272 mg QE/g of dry extract), and tannins (274.037 ± 11.3 mg CE/g of dry extract). The administration the hydroethanolic extract in two concentrations (1 or 2 g/kg) to male Swiss mice exhibited no marked adverse effects, as evidenced by serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) enzyme activity levels. In addition, the extract significantly reduced skin hyperplasia and inflammation induced by UV/sulfuric acid treatment as noted in tissue analyses and decreased protein expression of nuclear proliferation marker (Ki-67). This improvement was associated with a marked decrease in oxidative stress, as indicated by diminished lipid peroxidation levels, and restoration of the activity of endogenous antioxidant enzyme catalase (CAT) to control levels. Our findings demonstrated the potential of Orbea variegata hydroethanolic extract to be considered as a treatment for skin cancer, exhibiting its apparent safety and efficacy in reducing inflammation and carcinogenesis in a UV/sulfuric acid-induced Swiss mouse model, attributed to its phytochemical content and associated antioxidant activities.

Orbea variegata (L.) Haw 在皮肤癌中的作用:瑞士雄性小鼠体内模型研究的启示。
皮肤癌是最常见的一种恶性肿瘤,是公众健康的一大隐患。考虑到传统治疗方法存在诸多副作用,植物疗法可被视为一种可行的医疗替代方法。本研究旨在研究观赏植物 Orbea variegata (L.) Haw 在紫外线(UV)照射和硫酸处理联合诱导的动物模型中治疗皮肤癌的潜力。对 Orbea variegata 的水乙醇提取物进行了植物化学鉴定,通过定性筛选确定了还原糖、香豆素、生物碱、黄酮类、鞣质和皂苷的存在。定量分析显示,其中含有大量酚类化合物(29.435 ± 0.571 毫克 GAE/克干提取物)、黄酮类化合物(6.711 ± 0.272 毫克 QE/克干提取物)和单宁酸(274.037 ± 11.3 毫克 CE/克干提取物)。雄性瑞士小鼠服用两种浓度(1 或 2 克/千克)的水乙醇提取物后,血清中天门冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT)的酶活性水平未显示出明显的不良反应。此外,该提取物还能明显减少紫外线/硫酸处理引起的皮肤增生和炎症,这在组织分析和核增殖标志物(Ki-67)蛋白表达减少中均有体现。这种改善与氧化应激的明显降低有关,表现为脂质过氧化水平降低,内源性抗氧化酶过氧化氢酶(CAT)的活性恢复到控制水平。我们的研究结果表明,Orbea variegata 水乙醇提取物具有治疗皮肤癌的潜力,在紫外线/硫酸诱导的瑞士小鼠模型中,它在减少炎症和癌变方面具有明显的安全性和有效性,这归功于其植物化学成分含量和相关的抗氧化活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
5.20
自引率
19.20%
发文量
46
审稿时长
8-16 weeks
期刊介绍: The Journal of Toxicology and Environmental Health, Part A , Current Issues is an authoritative journal that features strictly refereed original research in the field of environmental sciences, public and occupational health, and toxicology.
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