{"title":"Evaluation of antioxidant enzyme levels, oxidative stress markers and serum prolidase activity in testicular cancer.","authors":"Mehmet Kaba, Sinan Kılıç, Halit Demir","doi":"10.1177/03915603241249232","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Testicular cancer is a significant malignancy affecting males, and understanding the underlying biochemical changes associated with the disease is essential for improved management and treatment strategies. Prolidase enzyme, has been implicated in various disease processes. The assessment of serum prolidase activity and its relationship with testicular cancer can provide valuable insights into the pathophysiology of the disease. The objective of this study was to investigate serum prolidase activity, oxidative stress markers, and antioxidant enzyme levels in patients with testicular cancer and evaluate their potential associations, aiming to enhance our understanding of the biochemical alterations and potential implications for testicular cancer management.</p><p><strong>Methods: </strong>A total of 33 male patients diagnosed with testicular cancer were included, along with 35 age-matched male volunteers as the control group. Serum samples were collected and stored at -20°C until analysis. The measurement of superoxide dismutase (SOD), glutathione peroxidase (GSHPx), glutathione-S-transferase (GST), malondialdehyde (MDA), glutathione (GSH), and prolidase levels was performed.</p><p><strong>Results: </strong>The findings demonstrated significantly elevated serum prolidase activity and malondialdehyde (MDA) levels in testicular cancer patients compared to the control group (all, <i>p</i> < 0.05). Conversely, superoxide dismutase (SOD), glutathione peroxidase (GSHPx), and glutathione-S-transferase (GST) levels were significantly lower in testicular cancer patients (<i>p</i> < 0.05).</p><p><strong>Conclusion: </strong>In this study, serum prolidase activity and biochemical markers associated with oxidative stress were investigated in testicular cancer patients. Oxidative stress markers and serum prolidase activity were found to be elevated in testicular cancer. Long-term prospective studies are needed to determine the effectiveness of antioxidant use in cancer treatment.</p>","PeriodicalId":23574,"journal":{"name":"Urologia Journal","volume":" ","pages":"807-812"},"PeriodicalIF":0.8000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Urologia Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1177/03915603241249232","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/5/14 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"UROLOGY & NEPHROLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Introduction: Testicular cancer is a significant malignancy affecting males, and understanding the underlying biochemical changes associated with the disease is essential for improved management and treatment strategies. Prolidase enzyme, has been implicated in various disease processes. The assessment of serum prolidase activity and its relationship with testicular cancer can provide valuable insights into the pathophysiology of the disease. The objective of this study was to investigate serum prolidase activity, oxidative stress markers, and antioxidant enzyme levels in patients with testicular cancer and evaluate their potential associations, aiming to enhance our understanding of the biochemical alterations and potential implications for testicular cancer management.
Methods: A total of 33 male patients diagnosed with testicular cancer were included, along with 35 age-matched male volunteers as the control group. Serum samples were collected and stored at -20°C until analysis. The measurement of superoxide dismutase (SOD), glutathione peroxidase (GSHPx), glutathione-S-transferase (GST), malondialdehyde (MDA), glutathione (GSH), and prolidase levels was performed.
Results: The findings demonstrated significantly elevated serum prolidase activity and malondialdehyde (MDA) levels in testicular cancer patients compared to the control group (all, p < 0.05). Conversely, superoxide dismutase (SOD), glutathione peroxidase (GSHPx), and glutathione-S-transferase (GST) levels were significantly lower in testicular cancer patients (p < 0.05).
Conclusion: In this study, serum prolidase activity and biochemical markers associated with oxidative stress were investigated in testicular cancer patients. Oxidative stress markers and serum prolidase activity were found to be elevated in testicular cancer. Long-term prospective studies are needed to determine the effectiveness of antioxidant use in cancer treatment.
简介睾丸癌是影响男性的重要恶性肿瘤，了解与该疾病相关的潜在生化变化对于改善管理和治疗策略至关重要。脯氨酸酶与多种疾病过程有关。评估血清丙烯酰酶活性及其与睾丸癌的关系可为了解该疾病的病理生理学提供有价值的信息。本研究旨在调查睾丸癌患者的血清绒毛膜促性腺激素酶活性、氧化应激标记物和抗氧化酶水平，并评估它们之间的潜在关联，从而加深我们对睾丸癌生化改变及其潜在影响的理解：方法：共纳入 33 名确诊为睾丸癌的男性患者，以及 35 名年龄匹配的男性志愿者作为对照组。采集血清样本并保存在零下 20 摄氏度的环境中，直至进行分析。对超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSHPx）、谷胱甘肽-S-转移酶（GST）、丙二醛（MDA）、谷胱甘肽（GSH）和绒毛膜促性腺激素酶水平进行了测定：结果：研究结果表明，与对照组相比，睾丸癌患者血清中的增殖酶活性和丙二醛（MDA）水平明显升高（均为 p p 结论：睾丸癌患者血清中的增殖酶活性和丙二醛（MDA）水平明显升高：本研究调查了睾丸癌患者的血清绒毛膜促性腺激素活性和与氧化应激相关的生化指标。结果发现，睾丸癌患者的氧化应激标记物和血清脯氨酸酶活性升高。需要进行长期的前瞻性研究，以确定抗氧化剂在癌症治疗中的应用效果。
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