Correlation of Serum and Gingival Crevicular Fluid Levels of Caspase-3 and Milk Fat Globule-Epidermal Growth Factor 8 on Gingival Health.

Q3 Dentistry
Indian Journal of Dental Research Pub Date : 2023-10-01 Epub Date: 2024-04-19 DOI:10.4103/ijdr.ijdr_62_23
Manonmani Pavithra Ravindran, A Geetha, Sathish Rajendran, Jaideep Mahendra, M Jyothi, Ambalavanan Namasivayam
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引用次数: 0

Abstract

Aim: This study aimed to estimate and correlate the serum and gingival crevicular fluid (GCF) levels of caspase-3 and milk fat globule-epidermal growth factor 8 (MFG-E8) in healthy, gingivitis and generalised chronic periodontitis subjects.

Materials and methods: A total of 24 subjects were selected and divided into three groups. After recording the periodontal parameters (plaque index (PI), modified gingival index (MGI), probing depth (PD) and clinical attachment level (CAL)), the serum and GCF samples were collected and the levels of caspase-3 and MFG-E8 were estimated using enzyme-linked immunoassay (ELISA).

Results: The mean values of PI, MGI, PD and CALs were significantly higher in group III when compared to group II and group I. The mean value of serum and GCF caspase-3 increased with increasing disease severity, whereas the mean serum and GCF values of MFG-E8 decreased with increasing severity of disease. Spearman's correlation showed a strong positive correlation between the serum and GCF levels of caspase-3 and periodontal parameters, whereas serum and GCF levels of MFG-E8 showed a strong negative correlation with the periodontal parameters.

Conclusion: The findings of this study are suggestive that the serum and GCF levels of caspase-3 and MFG-E8 could serve as a potential biomarker for the role of apoptosis in periodontal disease. However, further studies are required to explore the mechanism and understand the relationship between these apoptotic markers and periodontitis.

血清和牙龈蠕动液中 Caspase-3 和乳脂球-表皮生长因子 8 的水平与牙龈健康的相关性。
目的:本研究旨在评估健康、牙龈炎和全身慢性牙周炎受试者血清和牙龈细缝液(GCF)中caspase-3和乳脂球-表皮生长因子8(MFG-E8)的水平,并将其与之相关联:共选取 24 名受试者,分为三组。在记录牙周参数(牙菌斑指数(PI)、改良牙龈指数(MGI)、探诊深度(PD)和临床附着水平(CAL))后,收集血清和 GCF 样本,并使用酶联免疫分析法(ELISA)估算 caspase-3 和 MFG-E8 的水平:血清和GCF中caspase-3的平均值随着疾病严重程度的增加而增加,而血清和GCF中MFG-E8的平均值随着疾病严重程度的增加而减少。斯皮尔曼相关性表明,血清和 GCF 中的 caspase-3 水平与牙周参数之间存在很强的正相关性,而血清和 GCF 中的 MFG-E8 水平与牙周参数之间存在很强的负相关性:本研究结果表明,血清和GCF中的caspase-3和MFG-E8水平可作为牙周病中细胞凋亡作用的潜在生物标志物。然而,要探索这些凋亡标志物与牙周炎之间的机制和关系,还需要进一步的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Indian Journal of Dental Research
Indian Journal of Dental Research Dentistry-Dentistry (all)
CiteScore
1.80
自引率
0.00%
发文量
80
审稿时长
38 weeks
期刊介绍: Indian Journal of Dental Research (IJDR) is the official publication of the Indian Society for Dental Research (ISDR), India section of the International Association for Dental Research (IADR), published quarterly. IJDR publishes scientific papers on well designed and controlled original research involving orodental sciences. Papers may also include reports on unusual and interesting case presentations and invited review papers on significant topics.
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