Screening for metastasis-related genes in mouse melanoma cells through sequential tail vein injection.

Biophysics reports Pub Date : 2024-02-29 DOI:10.52601/bpr.2023.230043

Qinggang Hao, Rui Dong, Weiyu Bai, Dong Chang, Xinyi Yao, Yingru Zhang, Huangying Xu, Huiyan Li, Xiang Kui, Feng Wang, Yan Wang, Chengqin Wang, Yujie Lei, Yan Chen, Junling Shen, Lei Sang, Yan Bai, Jianwei Sun

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Abstract

Tumor metastasis, responsible for approximately 90% of cancer-associated mortality, remains poorly understood. Here in this study, we employed a melanoma lung metastasis model to screen for metastasis-related genes. By sequential tail vein injection of mouse melanoma B16F10 cells and the subsequently derived cells from lung metastasis into BALB/c mice, we successfully obtained highly metastatic B16F15 cells after five rounds of in vivo screening. RNA-sequencing analysis of B16F15 and B16F10 cells revealed a number of differentially expressed genes, some of these genes have previously been associated with tumor metastasis while others are novel discoveries. The identification of these metastasis-related genes not only improves our understanding of the metastasis mechanisms, but also provides potential diagnostic biomarkers and therapeutic targets for metastatic melanoma.

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通过连续尾静脉注射筛选小鼠黑色素瘤细胞中的转移相关基因。

肿瘤转移造成了约 90% 的癌症相关死亡率，但人们对肿瘤转移的了解仍然很少。在本研究中，我们利用黑色素瘤肺转移模型来筛选转移相关基因。通过将小鼠黑色素瘤 B16F10 细胞和随后从肺转移中获得的细胞依次尾静脉注射到 BALB/c 小鼠体内，经过五轮体内筛选，我们成功地获得了高转移性 B16F15 细胞。对 B16F15 和 B16F10 细胞进行的 RNA 序列分析发现了许多不同表达的基因，其中一些基因以前就与肿瘤转移有关，而另一些则是新发现的。这些转移相关基因的鉴定不仅提高了我们对转移机制的认识，还为转移性黑色素瘤提供了潜在的诊断生物标志物和治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biophysics reports

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8 weeks