Rou Zhang , Chuangchuang Xu , Yanghe Xie , Anqi Chen , Peicheng Lu , Minghui Wu , Guomin Han , Shunli Hu
{"title":"Biotransformation of aflatoxin B1 by a novel strain Brevundimonas sp. LF-1","authors":"Rou Zhang , Chuangchuang Xu , Yanghe Xie , Anqi Chen , Peicheng Lu , Minghui Wu , Guomin Han , Shunli Hu","doi":"10.1016/j.ibiod.2024.105810","DOIUrl":null,"url":null,"abstract":"<div><p>Aflatoxin B1 (AFB1) is a potent mycotoxin and a common source of food and feed contamination, posing risks to both human and animal health. The biotransformation of AFB1 has been proven to be a promising approach to control AFB1 contamination. In this study, <em>Brevundimonas</em> sp. LF-1 capable of degrading AFB1 was isolated from a corn-planted soil sample. Strain LF-1 could degrade 86.90% of 2 mg L<sup>−1</sup> AFB1 after incubation in Luria-Bertani (LB) medium at 30 °C for 72 h and exhibited high performance when exposed to up to 10.0 mg L<sup>−1</sup> AFB1. The optimum pH and temperature were 7.0–10.0 and 30 °C, respectively. The results of the <em>in vitro</em> cytotoxicity assay showed that the degradation products had considerably (<em>p</em> < 0.05) less harmful effects than the parent AFB1. Additionally, strain LF-1 possessed the bioremediation potential of AFB1 contamination for biocontrol strategies in animal fodder. Two putative novel AFB1-degrading enzymes, peroxiredoxin 1 and peroxiredoxin 2, were identified in the genome of strain LF-1. Comparative genomics indicated that the peroxiredoxin enzyme was widely distributed in the genus <em>Brevundimonas</em>. The comprehensive examination of strain LF-1 has outstanding potential for the development of detoxifying agents for AFB1 in the food and feed industries.</p></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":null,"pages":null},"PeriodicalIF":4.1000,"publicationDate":"2024-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Biodeterioration & Biodegradation","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0964830524000817","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Aflatoxin B1 (AFB1) is a potent mycotoxin and a common source of food and feed contamination, posing risks to both human and animal health. The biotransformation of AFB1 has been proven to be a promising approach to control AFB1 contamination. In this study, Brevundimonas sp. LF-1 capable of degrading AFB1 was isolated from a corn-planted soil sample. Strain LF-1 could degrade 86.90% of 2 mg L−1 AFB1 after incubation in Luria-Bertani (LB) medium at 30 °C for 72 h and exhibited high performance when exposed to up to 10.0 mg L−1 AFB1. The optimum pH and temperature were 7.0–10.0 and 30 °C, respectively. The results of the in vitro cytotoxicity assay showed that the degradation products had considerably (p < 0.05) less harmful effects than the parent AFB1. Additionally, strain LF-1 possessed the bioremediation potential of AFB1 contamination for biocontrol strategies in animal fodder. Two putative novel AFB1-degrading enzymes, peroxiredoxin 1 and peroxiredoxin 2, were identified in the genome of strain LF-1. Comparative genomics indicated that the peroxiredoxin enzyme was widely distributed in the genus Brevundimonas. The comprehensive examination of strain LF-1 has outstanding potential for the development of detoxifying agents for AFB1 in the food and feed industries.
期刊介绍:
International Biodeterioration and Biodegradation publishes original research papers and reviews on the biological causes of deterioration or degradation.