Isolation of a facultative methanotroph Methylocystis iwaonis SD4 from rice rhizosphere and establishment of rapid genetic tools for it.

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology Letters Pub Date : 2024-08-01 Epub Date: 2024-05-11 DOI:10.1007/s10529-024-03495-y

Yinghui Wang, Yuying Wang, Keyu Zhou, Haili Zhang, Minggen Cheng, Baozhan Wang, Xin Yan

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Abstract

Methanotrophs of the genus Methylocystis are frequently found in rice paddies. Although more than ten facultative methanotrophs have been reported since 2005, none of these strains was isolated from paddy soil. Here, a facultative methane-oxidizing bacterium, Methylocystis iwaonis SD4, was isolated and characterized from rhizosphere samples of rice plants in Nanjing, China. This strain grew well on methane or methanol but was able to grow slowly using acetate or ethanol. Moreover, strain SD4 showed sustained growth at low concentrations of methane (100 and 500 ppmv). M. iwaonis SD4 could utilize diverse nitrogen sources, including nitrate, urea, ammonium as well as dinitrogen. Strain SD4 possessed genes encoding both the particulate methane monooxygenase and the soluble methane monooxygenase. Simple and rapid genetic manipulation methods were established for this strain, enabling vector transformation and unmarked genetic manipulation. Fast growth rate and efficient genetic tools make M. iwaonis SD4 an ideal model to study facultative methanotrophs, and the ability to grow on low concentration of methane implies its potential in methane removal.

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从水稻根瘤中分离出一种兼性甲烷营养体 Methylocystis iwaonis SD4，并为其建立快速遗传工具。

水稻田中经常发现甲壳菌属的甲烷滋养菌。尽管自 2005 年以来已报道了十多种兼性甲烷营养菌，但没有一株是从稻田土壤中分离出来的。本文从中国南京的水稻根瘤样品中分离并鉴定了一种兼性甲烷氧化细菌--Methylocystis iwaonis SD4。该菌株在甲烷或甲醇中生长良好，但在醋酸或乙醇中生长缓慢。此外，菌株 SD4 在低浓度甲烷（100 和 500 ppmv）条件下也能持续生长。M. iwaonis SD4 能利用多种氮源，包括硝酸盐、尿素、铵和二氮。菌株 SD4 具有编码颗粒甲烷单加氧酶和可溶性甲烷单加氧酶的基因。针对该菌株建立了简单快速的遗传操作方法，可进行载体转化和无标记遗传操作。快速的生长速度和高效的遗传工具使 M. iwaonis SD4 成为研究兼性甲烷营养体的理想模型，而在低浓度甲烷中生长的能力则意味着其在甲烷去除方面的潜力。

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.