Yeast perilipin Pet10p/Pln1p interacts with Erg6p in ergosterol metabolism

IF 3.9 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Martina Garaiova , Yunfeng Ding , Roman Holic , Martin Valachovic , Congyan Zhang , Ivan Hapala , Pingsheng Liu
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Abstract

Lipid droplets (LD) are highly dynamic organelles specialized for the regulation of energy storage and cellular homeostasis. LD consist of a neutral lipid core surrounded by a phospholipid monolayer membrane with embedded proteins, most of which are involved in lipid homeostasis. In this study, we focused on one of the major LD proteins, sterol C24-methyltransferase, encoded by ERG6. We found that the absence of Erg6p resulted in an increased accumulation of yeast perilipin Pet10p in LD, while the disruption of PET10 was accompanied by Erg6p LD over-accumulation. An observed reciprocal enrichment of Erg6p and Pet10p in pet10Δ and erg6Δ mutants in LD, respectively, was related to specific functional changes in the LD and was not due to regulation on the expression level. The involvement of Pet10p in neutral lipid homeostasis was observed in experiments that focused on the dynamics of neutral lipid mobilization as time-dependent changes in the triacylglycerols (TAG) and steryl esters (SE) content. We found that the kinetics of SE hydrolysis was reduced in erg6Δ cells and the mobilization of SE was completely lost in mutants that lacked both Erg6p and Pet10p. In addition, we observed that decreased levels of SE in erg6Δpet10Δ was linked to an overexpression of steryl ester hydrolase Yeh1p. Lipid analysis of erg6Δpet10Δ showed that PET10 deletion altered the composition of ergosterol intermediates which had accumulated in erg6Δ. In conclusion, yeast perilipin Pet10p functionally interacts with Erg6p during the metabolism of ergosterol.

酵母围脂蛋白 Pet10p/Pln1p 在麦角甾醇代谢过程中与 Erg6p 相互作用。
脂滴(LD)是一种高度动态的细胞器,专门用于调节能量储存和细胞平衡。脂滴由中性脂质核心和磷脂单层膜组成,磷脂单层膜内嵌蛋白质,其中大多数参与脂质平衡。在这项研究中,我们重点研究了由 ERG6 编码的主要 LD 蛋白之一--甾醇 C24-甲基转移酶。我们发现,Erg6p的缺失导致酵母过脂蛋白Pet10p在LD中的积累增加,而PET10的破坏则伴随着Erg6p LD的过度积累。在 pet10Δ 和 erg6Δ 突变体中观察到的 Erg6p 和 Pet10p 在 LD 中的相互富集分别与 LD 中的特定功能变化有关,而不是由于表达水平的调节。Pet10p 参与了中性脂质平衡,实验重点是中性脂质动员的动态变化,即三酰基甘油(TAG)和固醇酯(SE)含量随时间的变化。我们发现,在 Erg6Δ 细胞中,SE 的水解动力学降低,而在同时缺乏 Erg6p 和 Pet10p 的突变体中,SE 的动员完全丧失。此外,我们还观察到,erg6Δpet10Δ 中 SE 水平的降低与甾基酯水解酶 Yeh1p 的过表达有关。对erg6Δpet10Δ的脂质分析表明,PET10的缺失改变了erg6Δ中积累的麦角甾醇中间产物的组成。总之,酵母围脂蛋白 Pet10p 在麦角固醇代谢过程中与 Erg6p 发生了功能性相互作用。
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来源期刊
CiteScore
11.00
自引率
2.10%
发文量
109
审稿时长
53 days
期刊介绍: BBA Molecular and Cell Biology of Lipids publishes papers on original research dealing with novel aspects of molecular genetics related to the lipidome, the biosynthesis of lipids, the role of lipids in cells and whole organisms, the regulation of lipid metabolism and function, and lipidomics in all organisms. Manuscripts should significantly advance the understanding of the molecular mechanisms underlying biological processes in which lipids are involved. Papers detailing novel methodology must report significant biochemical, molecular, or functional insight in the area of lipids.
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