Characterization and expression profiling of buffalo IFN-lambda family

IF 1.4 3区农林科学 Q4 IMMUNOLOGY

Veterinary immunology and immunopathology Pub Date : 2024-05-04 DOI:10.1016/j.vetimm.2024.110770

Devika Gautam , Anil Sindhu , Ashutosh Vats , Shiveeli Rajput , Mayank Roshan , Hanshika Pal , Sachinandan De

{"title":"Characterization and expression profiling of buffalo IFN-lambda family","authors":"Devika Gautam , Anil Sindhu , Ashutosh Vats , Shiveeli Rajput , Mayank Roshan , Hanshika Pal , Sachinandan De","doi":"10.1016/j.vetimm.2024.110770","DOIUrl":null,"url":null,"abstract":"<div><p>Interferon lambda (IFN-λ) is an important type III interferon triggered mainly by viral infection. IFN-λ binds to their heterodimeric receptors and signals through JAK-STAT pathways similar to type I IFN. In this study, we deduced the buffalo IFN-λ sequences through the polymerase chain reaction, and then studied IFN-λ’s expression patterns in different tissues, and post induction with poly I:C and live MRSA using RT-qPCR. The full-length sequences of buffalo <em>IFN-λ3</em>, IFN-λ receptors, and a transcript variant of IFN-λ4 were determined. IFN-λ1 is identified as a pseudogene. Virus response elements and a recombination hotspot factor was observed in the regulatory region of IFN-λ. The IFN-λ3 expressed highest in lungs and monocytes but IFN-λ4 did not. The expression of Interferon Lambda Receptor 1 was tissue specific, while Interleukin 10 Receptor subunit beta was ubiquitous. Following poly I:C induction, IFN-λ3 expression was primarily observed in epithelial cells as opposed to fibroblasts, displaying cell type-dependent expression. The cytosolic RNA sensors were expressed highest in endometrial epithelial cells, whereas the endosomal receptor was higher in fibroblasts. 2’,5’-oligoadenylate synthetase expressed higher in fibroblasts, myxoma resistance protein 1 and IFN-stimulated gene 56 in epithelial cells, displaying cell-specific antiviral response of the interferon stimulated genes (ISGs). The endometrial epithelial cells expressed IFN-λ3 after live <em>S. aureus</em> infection indicating its importance in bacterial infection. The induction of IFN-λ3 was <em>S. aureus</em> isolate specific at the same multiplicity of infection (MOI). This study elucidates the IFN-λ sequences, diverse expression patterns revealing tissue specificity, and specificity in response to poly I:C and bacterial stimuli, emphasising its crucial role in innate immune response modulation.</p></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"272 ","pages":"Article 110770"},"PeriodicalIF":1.4000,"publicationDate":"2024-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary immunology and immunopathology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0165242724000564","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"IMMUNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Interferon lambda (IFN-λ) is an important type III interferon triggered mainly by viral infection. IFN-λ binds to their heterodimeric receptors and signals through JAK-STAT pathways similar to type I IFN. In this study, we deduced the buffalo IFN-λ sequences through the polymerase chain reaction, and then studied IFN-λ’s expression patterns in different tissues, and post induction with poly I:C and live MRSA using RT-qPCR. The full-length sequences of buffalo IFN-λ3, IFN-λ receptors, and a transcript variant of IFN-λ4 were determined. IFN-λ1 is identified as a pseudogene. Virus response elements and a recombination hotspot factor was observed in the regulatory region of IFN-λ. The IFN-λ3 expressed highest in lungs and monocytes but IFN-λ4 did not. The expression of Interferon Lambda Receptor 1 was tissue specific, while Interleukin 10 Receptor subunit beta was ubiquitous. Following poly I:C induction, IFN-λ3 expression was primarily observed in epithelial cells as opposed to fibroblasts, displaying cell type-dependent expression. The cytosolic RNA sensors were expressed highest in endometrial epithelial cells, whereas the endosomal receptor was higher in fibroblasts. 2’,5’-oligoadenylate synthetase expressed higher in fibroblasts, myxoma resistance protein 1 and IFN-stimulated gene 56 in epithelial cells, displaying cell-specific antiviral response of the interferon stimulated genes (ISGs). The endometrial epithelial cells expressed IFN-λ3 after live S. aureus infection indicating its importance in bacterial infection. The induction of IFN-λ3 was S. aureus isolate specific at the same multiplicity of infection (MOI). This study elucidates the IFN-λ sequences, diverse expression patterns revealing tissue specificity, and specificity in response to poly I:C and bacterial stimuli, emphasising its crucial role in innate immune response modulation.

查看原文本刊更多论文

水牛 IFN-lambda 家族的特征和表达谱分析

λ型干扰素（IFN-λ）是一种重要的 III 型干扰素，主要由病毒感染引发。IFN-λ 与它们的异源二聚体受体结合，并通过 JAK-STAT 通路发出信号，这与 I 型 IFN 相似。在这项研究中，我们通过聚合酶链式反应推导出了水牛 IFN-λ 的序列，然后利用 RT-qPCR 研究了 IFN-λ 在不同组织中的表达模式，以及在聚 I:C 和活 MRSA 诱导后的表达模式。测定了水牛 IFN-λ3、IFN-λ受体和 IFN-λ4 转录本变体的全长序列。IFN-λ1 被鉴定为假基因。在 IFN-λ 的调控区域观察到了病毒反应元件和重组热点因子。IFN-λ3 在肺和单核细胞中表达量最高，但 IFN-λ4 却没有。干扰素-λ受体 1 的表达具有组织特异性，而白细胞介素 10 受体亚基 beta 则无处不在。经 poly I:C 诱导后，IFN-λ3 主要在上皮细胞而非成纤维细胞中表达，显示出细胞类型依赖性。子宫内膜上皮细胞中细胞膜 RNA 传感器的表达量最高，而成纤维细胞中内膜受体的表达量较高。2',5'-醇溶腺苷酸合成酶在成纤维细胞中表达较高，肌瘤抗性蛋白 1 和 IFN 刺激基因 56 在上皮细胞中表达较高，这表明干扰素刺激基因（ISGs）的抗病毒反应具有细胞特异性。金黄色葡萄球菌活菌感染后，子宫内膜上皮细胞表达了 IFN-λ3，表明其在细菌感染中的重要性。在相同感染倍数（MOI）下，IFN-λ3的诱导具有金黄色葡萄球菌分离株特异性。这项研究阐明了 IFN-λ 序列、显示组织特异性的多种表达模式以及对聚 I:C 和细菌刺激的特异性，强调了它在先天性免疫反应调节中的关键作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Veterinary immunology and immunopathology 农林科学-免疫学

CiteScore

3.40

自引率

5.60%

发文量

审稿时长

70 days

期刊介绍： The journal reports basic, comparative and clinical immunology as they pertain to the animal species designated here: livestock, poultry, and fish species that are major food animals and companion animals such as cats, dogs, horses and camels, and wildlife species that act as reservoirs for food, companion or human infectious diseases, or as models for human disease. Rodent models of infectious diseases that are of importance in the animal species indicated above,when the disease requires a level of containment that is not readily available for larger animal experimentation (ABSL3), will be considered. Papers on rabbits, lizards, guinea pigs, badgers, armadillos, elephants, antelope, and buffalo will be reviewed if the research advances our fundamental understanding of immunology, or if they act as a reservoir of infectious disease for the primary animal species designated above, or for humans. Manuscripts employing other species will be reviewed if justified as fitting into the categories above. The following topics are appropriate: biology of cells and mechanisms of the immune system, immunochemistry, immunodeficiencies, immunodiagnosis, immunogenetics, immunopathology, immunology of infectious disease and tumors, immunoprophylaxis including vaccine development and delivery, immunological aspects of pregnancy including passive immunity, autoimmuity, neuroimmunology, and transplanatation immunology. Manuscripts that describe new genes and development of tools such as monoclonal antibodies are also of interest when part of a larger biological study. Studies employing extracts or constituents (plant extracts, feed additives or microbiome) must be sufficiently defined to be reproduced in other laboratories and also provide evidence for possible mechanisms and not simply show an effect on the immune system.