Detection and identification of circulating tumor cells in parathyroid tumors and correlation analysis with clinicopathological features.

IF 3.7 3区医学 Q2 Medicine

Endocrine Pub Date : 2024-09-01 Epub Date: 2024-05-10 DOI:10.1007/s12020-024-03831-2

Jiacheng Wang, Xingran Jiang, Qian Wang, Teng Zhao, Hong Shen, Xing Liu, Dalin Feng, Rongfang Shen, Yuting Wang, Wenjing Yang, Bojun Wei

{"title":"Detection and identification of circulating tumor cells in parathyroid tumors and correlation analysis with clinicopathological features.","authors":"Jiacheng Wang, Xingran Jiang, Qian Wang, Teng Zhao, Hong Shen, Xing Liu, Dalin Feng, Rongfang Shen, Yuting Wang, Wenjing Yang, Bojun Wei","doi":"10.1007/s12020-024-03831-2","DOIUrl":null,"url":null,"abstract":"Introduction: The differential diagnosis of parathyroid carcinoma (PC)/parathyroid adenoma (PA) in parathyroid tumors is critical for their management and prognosis. Circulating tumor cells (CTCs) identification in the peripheral blood of parathyroid tumors remains unknown. In this study, we proposed to investigate the differences of CTCs in PC/PA and the relationship with clinicopathologic features to assess its relevance to PC and value in identifying PC/PA.Methods and materials: Peripheral blood was collected from 27 patients with PC and 37 patients with PA treated in our hospital, and the number of chromosome 8 aberrant CTCs was detected by negative magnetic bead sorting fluorescence in situ hybridization (NE-FISH). The differences of CTCs in PC/PA peripheral blood were compared and their diagnostic efficacy was evaluated, and the correlation between CTCs and clinicopathological features of PC was further explored.Results: CTCs differed significantly in PC/PA (p = 0.0008) and were up-regulated in PC, with good diagnostic efficacy. CTCs combined with alkaline phosphatase (ALP) assay improved the diagnostic efficacy in identifying PC/PA (AUC = 0.7838, p = 0.0001). The number of CTCs was correlated with tumor dimensions, but not significantly correlated with clinical markers such as calcium and PTH and pathological features such as vascular invasion, lymph node metastasis and distant metastasis.Conclusion: As a non-invasive liquid biopsy method, CTCs test combined with ALP test can be used as an important reference basis for timely and accurate identification and treatment of PC. It is of great significance to improve the current situation of PC diagnosis, treatment and prognosis.","PeriodicalId":11572,"journal":{"name":"Endocrine","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Endocrine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s12020-024-03831-2","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/5/10 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction: The differential diagnosis of parathyroid carcinoma (PC)/parathyroid adenoma (PA) in parathyroid tumors is critical for their management and prognosis. Circulating tumor cells (CTCs) identification in the peripheral blood of parathyroid tumors remains unknown. In this study, we proposed to investigate the differences of CTCs in PC/PA and the relationship with clinicopathologic features to assess its relevance to PC and value in identifying PC/PA.

Methods and materials: Peripheral blood was collected from 27 patients with PC and 37 patients with PA treated in our hospital, and the number of chromosome 8 aberrant CTCs was detected by negative magnetic bead sorting fluorescence in situ hybridization (NE-FISH). The differences of CTCs in PC/PA peripheral blood were compared and their diagnostic efficacy was evaluated, and the correlation between CTCs and clinicopathological features of PC was further explored.

Results: CTCs differed significantly in PC/PA (p = 0.0008) and were up-regulated in PC, with good diagnostic efficacy. CTCs combined with alkaline phosphatase (ALP) assay improved the diagnostic efficacy in identifying PC/PA (AUC = 0.7838, p = 0.0001). The number of CTCs was correlated with tumor dimensions, but not significantly correlated with clinical markers such as calcium and PTH and pathological features such as vascular invasion, lymph node metastasis and distant metastasis.

Conclusion: As a non-invasive liquid biopsy method, CTCs test combined with ALP test can be used as an important reference basis for timely and accurate identification and treatment of PC. It is of great significance to improve the current situation of PC diagnosis, treatment and prognosis.

Abstract Image

查看原文本刊更多论文

甲状旁腺肿瘤循环肿瘤细胞的检测和鉴定以及与临床病理特征的相关分析

导言：甲状旁腺肿瘤中甲状旁腺癌（PC）/甲状旁腺腺瘤（PA）的鉴别诊断对于甲状旁腺肿瘤的治疗和预后至关重要。甲状旁腺肿瘤患者外周血中循环肿瘤细胞（CTCs）的鉴定仍是未知数。在本研究中，我们拟探讨CTCs在PC/PA中的差异及其与临床病理特征的关系，以评估其与PC的相关性以及在鉴别PC/PA中的价值：收集本院收治的27例PC患者和37例PA患者的外周血，通过阴性磁珠分选荧光原位杂交（NE-FISH）检测8号染色体畸变CTC的数量。比较了PC/PA外周血中CTCs的差异，评估了其诊断效果，并进一步探讨了CTCs与PC临床病理特征的相关性：结果：CTCs在PC/PA中差异明显（p = 0.0008），在PC中呈上调状态，具有良好的诊断效果。CTCs与碱性磷酸酶（ALP）测定相结合，提高了鉴别PC/PA的诊断效果（AUC = 0.7838，p = 0.0001）。CTCs数量与肿瘤尺寸相关，但与钙和PTH等临床指标以及血管侵犯、淋巴结转移和远处转移等病理特征无明显相关性：结论：作为一种无创液体活检方法，CTCs检测与ALP检测相结合，可作为及时、准确鉴别和治疗PC的重要参考依据。结论：CTCs检测作为一种无创液体活检方法，结合ALP检测可作为及时、准确鉴别和治疗PC的重要参考依据，对改善PC的诊断、治疗和预后现状具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Endocrine 医学-内分泌学与代谢

CiteScore

6.40

自引率

5.40%

发文量

期刊介绍： Well-established as a major journal in today’s rapidly advancing experimental and clinical research areas, Endocrine publishes original articles devoted to basic (including molecular, cellular and physiological studies), translational and clinical research in all the different fields of endocrinology and metabolism. Articles will be accepted based on peer-reviews, priority, and editorial decision. Invited reviews, mini-reviews and viewpoints on relevant pathophysiological and clinical topics, as well as Editorials on articles appearing in the Journal, are published. Unsolicited Editorials will be evaluated by the editorial team. Outcomes of scientific meetings, as well as guidelines and position statements, may be submitted. The Journal also considers special feature articles in the field of endocrine genetics and epigenetics, as well as articles devoted to novel methods and techniques in endocrinology. Endocrine covers controversial, clinical endocrine issues. Meta-analyses on endocrine and metabolic topics are also accepted. Descriptions of single clinical cases and/or small patients studies are not published unless of exceptional interest. However, reports of novel imaging studies and endocrine side effects in single patients may be considered. Research letters and letters to the editor related or unrelated to recently published articles can be submitted. Endocrine covers leading topics in endocrinology such as neuroendocrinology, pituitary and hypothalamic peptides, thyroid physiological and clinical aspects, bone and mineral metabolism and osteoporosis, obesity, lipid and energy metabolism and food intake control, insulin, Type 1 and Type 2 diabetes, hormones of male and female reproduction, adrenal diseases pediatric and geriatric endocrinology, endocrine hypertension and endocrine oncology.