A Novel Near-Infrared Fluorescent Probe for the Detection of Endogenous Peroxynitrite (ONOO^-) in Living Cells.

IF 2.6 4区化学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Fluorescence Pub Date : 2025-05-01 Epub Date: 2024-05-09 DOI:10.1007/s10895-024-03733-6

Jinting Shang, Wanxia Gao, Junluan Cai, Yan Yang, Chen Wang, Na Zhao, Haiping Wang, Yibin Zhang

引用次数: 0

Abstract

In this study, we present a novel near-infrared (NIR) fluorescent probe Nile-ONO designed for the selective and sensitive detection of ONOO^-. The probe Nile-ONO employed Nile red as the fluorophore, with diphenylphosphinate serving as the reaction site. In the presence of ONOO^-, the probe Nile-ONO exhibits remarkable fluorescence enhancement at 659 nm, with a response time of less than 20 min and a low detection limit of 0.32 µM. Importantly, MTT assays demonstrate low cytotoxicity in living cells. Furthermore, Nile-ONO has excellent imaging capabilities for endogenous ONOO^-. Overall, this work introduces a valuable new method for the rapid detection of ONOO^- in biological systems.

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用于检测活细胞中内源性过亚硝酸盐（ONOO-）的新型近红外荧光探针。

在这项研究中，我们提出了一种新型近红外（NIR）荧光探针 Nile-ONO，用于选择性和灵敏地检测 ONOO-。探针 Nile-ONO 采用尼罗河红作为荧光团，二苯基膦酸盐作为反应位点。在 ONOO- 存在的情况下，探针 Nile-ONO 在 659 纳米波长处显示出显著的荧光增强，响应时间小于 20 分钟，检测限低至 0.32 µM。重要的是，MTT 分析表明其在活细胞中的细胞毒性很低。此外，Nile-ONO 对内源性 ONOO- 具有出色的成像能力。总之，这项工作为快速检测生物系统中的 ONOO- 引入了一种有价值的新方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Fluorescence 化学-分析化学

CiteScore

4.60

自引率

7.40%

发文量

203

审稿时长

5.4 months

期刊介绍： Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.

A Novel Near-Infrared Fluorescent Probe for the Detection of Endogenous Peroxynitrite (ONOO-) in Living Cells.

Abstract

A Novel Near-Infrared Fluorescent Probe for the Detection of Endogenous Peroxynitrite (ONOO^-) in Living Cells.