Biomechanical and histological comparison of two suture configurations for soft tissue grafts: speedtrap™ versus krackow stitch.

IF 1.4 4区 医学 Q4 CELL BIOLOGY
Cell and Tissue Banking Pub Date : 2024-06-01 Epub Date: 2024-05-10 DOI:10.1007/s10561-024-10133-4
Anell Olivos-Meza, Edgar Manuel Hernández-Espinoza, Víctor Manuel Domínguez-Hernández, Víctor Manuel Araujo-Monsalvo, Eréndira Georgina Estrada-Villaseñor, Raúl Pichardo-Bahena, Luis Ángel Murguía-González, Juan Diego Rodríguez-Reinoso, Jonatan de Jesus Hernández-León, Carlos Landa-Solis
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引用次数: 0

Abstract

To compare 2 different graft preparation techniques to determine biomechanical strength and resultant tissue trauma evaluated by histology. Twelve common flexors of the finger's tendons were prepared with either tubulization (SpeedTrap™) or transtendon stiches (Orthocord™). The stiffness, resistance and energy at maximum load were tested for biomechanical assessment in both groups. After load testing, Samples were stained with hematoxylin and eosin (HE) to evaluate histological damage. We observe that the time to prepare tendons with SpeedTrap™ was 8.3 times faster (1:25 min) than traditional ones (15:02 min). In all cases, the mean values for SpeedTrap™ were higher in terms of strength, stiffness and energy at maximum load than for traditional suture but without significant difference (p > 0.05). The Krackow stitch produces greater structural damage to the collagen fibers while SpeedTrap™ maintains better organized arrangement of the fibers after tubulization preparation. With the results obtained, we can conclude that the tubulization technique allows faster graft preparation with less structural damage to the manipulated tissue without altering the biomechanical resistance provided by the transtendon suture technique.

Abstract Image

用于软组织移植的两种缝合配置的生物力学和组织学比较:Speedtrap™ 与 Krackow 缝合。
比较两种不同的移植准备技术,以确定生物力学强度和组织学评估的组织创伤结果。用管状法(SpeedTrap™)或经肌腱缝合法(Orthocord™)制备了 12 根手指的常见屈肌腱。对两组肌腱的刚度、阻力和最大负荷时的能量进行了生物力学评估测试。负荷测试后,用苏木精和伊红(HE)对样本进行染色,以评估组织损伤情况。我们发现,使用 SpeedTrap™ 制备肌腱的时间(1:25 分钟)比传统方法(15:02 分钟)快 8.3 倍。在所有情况下,SpeedTrap™ 在强度、刚度和最大负荷能量方面的平均值均高于传统缝合线,但差异不显著(P > 0.05)。Krackow 缝合线对胶原纤维的结构损伤更大,而 SpeedTrap™ 在管化制备后能更好地保持纤维的有序排列。根据所获得的结果,我们可以得出结论,管化技术可以更快地进行移植物制备,对所操作组织的结构损伤较小,同时不会改变经腱缝合技术所提供的生物力学阻力。
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来源期刊
Cell and Tissue Banking
Cell and Tissue Banking CELL BIOLOGY-ENGINEERING, BIOMEDICAL
CiteScore
3.10
自引率
13.30%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Cell and Tissue Banking provides a forum for disseminating information to scientists and clinicians involved in the banking and transplantation of cells and tissues. Cell and Tissue Banking is an international, peer-reviewed journal that publishes original papers in the following areas: basic research concerning general aspects of tissue banking such as quality assurance and control of banked cells/tissues, effects of preservation and sterilisation methods on cells/tissues, biotechnology, etc.; clinical applications of banked cells/tissues; standards of practice in procurement, processing, storage and distribution of cells/tissues; ethical issues; medico-legal issues.
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