PCDHGA10 as a Potential Biomarker of Lung Squamous Cell Carcinoma Based on Bioinformatics and Experimental Verification.

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Biotechnology Pub Date : 2025-05-01 Epub Date: 2024-05-10 DOI:10.1007/s12033-024-01178-7

Shuming Song, Ruijiao Lu, Yuanya Chen, Yangchun Feng

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引用次数: 0

Abstract

Procalcitonin gamma subfamily A, 10 (PCDHGA10) is a member of the procalcitonin gamma gene cluster, which is associated with neuronal synapse development. However, there are lack of studies on the role and potential prognostic value of PCDHGA10 in lung squamous cell carcinoma (LUSC). We analyzed the RNAseq data of PCDHGA10 to compare their expression differences. Then survival analysis, tumor microenvironment (TME) analysis, and mutation analysis were carried out. Additionally, we performed gene ontology (GO) and kyoto gene encyclopedia (KEGG) enrichment analyses to explore potential signal pathways. PCDHGA10 protein expression was evaluated using immunohistochemistry (IHC) on tissue microarrays (HLugS180Su02). By microarray analysis and database analysis, we found that PCDHGA10 was significantly highly expressed in LUSC. Sufferers with elevated PCDHGA10 levels exhibited a worse prognosis, according to the survival analysis. The PCDHGA10 mutation was also linked to LUSC patient prognosis. Besides, PCDHGA10 was closely related to tumor immune cell infiltration and immune checkpoints. In conclusion, PCDHGA10 is expected to become a new molecular marker for LUSC.

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基于生物信息学和实验验证的肺鳞癌潜在生物标记物 PCDHGA10

降钙素原γ亚家族A，10（PCDHGA10）是降钙素原γ基因簇的成员，与神经元突触的发育有关。然而，目前还缺乏有关 PCDHGA10 在肺鳞癌（LUSC）中的作用和潜在预后价值的研究。我们分析了 PCDHGA10 的 RNAseq 数据，比较它们的表达差异。然后进行了生存分析、肿瘤微环境（TME）分析和突变分析。此外，我们还进行了基因本体（GO）和京都基因百科全书（KEGG）富集分析，以探索潜在的信号通路。通过组织芯片（HLugS180Su02）上的免疫组织化学（IHC）评估了PCDHGA10蛋白的表达。通过微阵列分析和数据库分析，我们发现 PCDHGA10 在淋巴结核中明显高表达。根据生存分析，PCDHGA10水平升高的患者预后较差。PCDHGA10突变也与LUSC患者的预后有关。此外，PCDHGA10还与肿瘤免疫细胞浸润和免疫检查点密切相关。总之，PCDHGA10有望成为LUSC新的分子标记物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Biotechnology 医学-生化与分子生物学

CiteScore

4.10

自引率

3.80%

发文量

165

审稿时长

6 months

期刊介绍： Molecular Biotechnology publishes original research papers on the application of molecular biology to both basic and applied research in the field of biotechnology. Particular areas of interest include the following: stability and expression of cloned gene products, cell transformation, gene cloning systems and the production of recombinant proteins, protein purification and analysis, transgenic species, developmental biology, mutation analysis, the applications of DNA fingerprinting, RNA interference, and PCR technology, microarray technology, proteomics, mass spectrometry, bioinformatics, plant molecular biology, microbial genetics, gene probes and the diagnosis of disease, pharmaceutical and health care products, therapeutic agents, vaccines, gene targeting, gene therapy, stem cell technology and tissue engineering, antisense technology, protein engineering and enzyme technology, monoclonal antibodies, glycobiology and glycomics, and agricultural biotechnology.