LncRNA LINC00466 Promotes the Progression of Breast Cancer via miR-4731-5p/EPHA2 Pathway.

IF 2.2 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current pharmaceutical biotechnology Pub Date : 2024-05-09 DOI:10.2174/0113892010290582240419051056

Xue Han, Fan Shi, Shujun Guo, Yao Li, Hongtao Wang, Chuanwang Song, Shiwu Wu

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引用次数: 0

Abstract

Background: Breast Cancer (BC) is a female malignancy with a high mortality rate. Novel diagnostic and prognostic biomarkers are valuable for reducing BC mortality. Our study is designed to undrape the precise role of the LINC00466/miR-4731-5p/EPHA2 axis in BC.

Methods: The Cancer Genome Atlas (TCGA) sequencing dataset was utilized to compare the levels of LINC00466. The levels of LINC00466, miR-4731-5p, and EPHA2 were tested by qRTPCR. Cell proliferation and cycle were detected by CCK-8 assay and flow cytometer. In vivo role of LINC00466 was tested by Xenograft nude models. Binding sites were predicted by TargetScan and Starbase. The binding relationship was employed by Dual-luciferase reporter gene assay and RNA pull-down assay.

Results: LINC00466 was increased in human breast cancer tissues. LINC00466 was negatively associated with miR-4731-5p and positively correlated with EPHA2 in human breast cancer tissues. Down-regulation of LINC00466 suppressed the proliferation and arrested the cell cycle of breast cancer cells, and inhibited tumor growth in vivo.

Conclusion: LINC00466 promoted BC development via mediating the miR-4731-5p/EPHA2 axis, which has the potential value as a promising therapeutic target in BC.

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LncRNA LINC00466通过miR-4731-5p/EPHA2途径促进乳腺癌的进展

背景：乳腺癌（BC）是死亡率很高的女性恶性肿瘤。新的诊断和预后生物标志物对降低乳腺癌死亡率很有价值。我们的研究旨在揭示 LINC00466/miR-4731-5p/EPHA2 轴在乳腺癌中的确切作用。研究方法利用癌症基因组图谱（TCGA）测序数据集来比较LINC00466的水平。通过 qRTPCR 检测 LINC00466、miR-4731-5p 和 EPHA2 的水平。通过 CCK-8 检测法和流式细胞仪检测细胞增殖和周期。LINC00466 的体内作用通过裸鼠异种移植模型进行检测。利用 TargetScan 和 Starbase 预测了结合位点。通过双荧光素酶报告基因检测和 RNA 拉取检测确定了结合关系。结果人类乳腺癌组织中 LINC00466 的含量增加。在人类乳腺癌组织中，LINC00466 与 miR-4731-5p 呈负相关，与 EPHA2 呈正相关。下调 LINC00466 能抑制乳腺癌细胞的增殖和细胞周期的停止，并能抑制肿瘤在体内的生长。结论LINC00466通过介导miR-4731-5p/EPHA2轴促进乳腺癌的发展，具有作为乳腺癌治疗靶点的潜在价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current pharmaceutical biotechnology 医学-生化与分子生物学

CiteScore

5.60

自引率

3.60%

发文量

203

审稿时长

6 months

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