Effects of transcription factor SOX11 on the biological behavior of neuroblastoma cell and potential regulatory mechanism.

IF 1.2 4区医学 Q3 SURGERY

Annals of Surgical Treatment and Research Pub Date : 2024-05-01 Epub Date: 2024-04-30 DOI:10.4174/astr.2024.106.5.284

Jing-Ru Huang, Yong Li, Peng Chen, Ji-Xiu Wei, Xia Yang, Qiong-Qian Xu, Jia-Bo Chen

{"title":"Effects of transcription factor SOX11 on the biological behavior of neuroblastoma cell and potential regulatory mechanism.","authors":"Jing-Ru Huang, Yong Li, Peng Chen, Ji-Xiu Wei, Xia Yang, Qiong-Qian Xu, Jia-Bo Chen","doi":"10.4174/astr.2024.106.5.284","DOIUrl":null,"url":null,"abstract":"Purpose: This study aimed to analyze the expression and prognosis of SRY-box transcription factor 11 (SOX11) in neuroblastoma (NB), as well as the biological function and potential regulatory mechanism of SOX11 in NB.Methods: Public RNA sequencing was used to detect the expression level of SOX11. The Kaplan-Meier curve and hazard ratios (HR) were used to determine the prognostic value of SOX11 in NB. Functional analyses were performed using CCK8, wound healing assay, and transwell invasion assay. Finally, the potential target genes of SOX11 were predicted by Harmonizonme (Ma'ayan Laboratory) and Cistrome Data Browser (Cistrome Project) database to explore the potential molecular mechanism of SOX11 in NB.Results: Compared with normal adrenal tissue, the expression of SOX11 in NB tissue was significantly upregulated. The Kaplan-Meier curve showed that high expression of SOX11 was associated with poor prognosis in children with NB (HR, 1.719; P = 0.049). SOX11 knockdown suppressed the migration capacity of SK-N-SH cells but did not affect proliferation and invasion capacity. Enhancer of zeste homolog 2 (EZH2) may be a potential downstream target gene for the transcription factor SOX11 to play a role in NB.Conclusion: The transcription factor SOX11 was significantly upregulated in NB. SOX11 knockdown suppressed the migration capacity of NB cell SK-N-SH. SOX11 may promote the progression of NB by targeting EZH2.","PeriodicalId":8071,"journal":{"name":"Annals of Surgical Treatment and Research","volume":null,"pages":null},"PeriodicalIF":1.2000,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11076950/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annals of Surgical Treatment and Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.4174/astr.2024.106.5.284","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/4/30 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"SURGERY","Score":null,"Total":0}

引用次数: 0

Abstract

Purpose: This study aimed to analyze the expression and prognosis of SRY-box transcription factor 11 (SOX11) in neuroblastoma (NB), as well as the biological function and potential regulatory mechanism of SOX11 in NB.

Methods: Public RNA sequencing was used to detect the expression level of SOX11. The Kaplan-Meier curve and hazard ratios (HR) were used to determine the prognostic value of SOX11 in NB. Functional analyses were performed using CCK8, wound healing assay, and transwell invasion assay. Finally, the potential target genes of SOX11 were predicted by Harmonizonme (Ma'ayan Laboratory) and Cistrome Data Browser (Cistrome Project) database to explore the potential molecular mechanism of SOX11 in NB.

Results: Compared with normal adrenal tissue, the expression of SOX11 in NB tissue was significantly upregulated. The Kaplan-Meier curve showed that high expression of SOX11 was associated with poor prognosis in children with NB (HR, 1.719; P = 0.049). SOX11 knockdown suppressed the migration capacity of SK-N-SH cells but did not affect proliferation and invasion capacity. Enhancer of zeste homolog 2 (EZH2) may be a potential downstream target gene for the transcription factor SOX11 to play a role in NB.

Conclusion: The transcription factor SOX11 was significantly upregulated in NB. SOX11 knockdown suppressed the migration capacity of NB cell SK-N-SH. SOX11 may promote the progression of NB by targeting EZH2.

查看原文本刊更多论文

转录因子 SOX11 对神经母细胞瘤细胞生物学行为的影响及潜在调控机制

目的：本研究旨在分析SRY-盒转录因子11（SOX11）在神经母细胞瘤（NB）中的表达和预后，以及SOX11在NB中的生物学功能和潜在调控机制：方法：采用公共RNA测序检测SOX11的表达水平。方法：采用公共RNA测序检测SOX11的表达水平，并利用Kaplan-Meier曲线和危险比（HR）确定SOX11在NB中的预后价值。利用 CCK8、伤口愈合试验和经孔侵袭试验进行了功能分析。最后，通过Harmonizonme（Ma'ayan实验室）和Cistrome Data Browser（Cistrome项目）数据库预测了SOX11的潜在靶基因，以探索SOX11在NB中的潜在分子机制：结果：与正常肾上腺组织相比，SOX11在NB组织中的表达明显上调。Kaplan-Meier曲线显示，SOX11的高表达与NB患儿的不良预后相关（HR，1.719；P = 0.049）。SOX11基因敲除抑制了SK-N-SH细胞的迁移能力，但不影响其增殖和侵袭能力。泽斯特同源增强子2（EZH2）可能是转录因子SOX11在NB中发挥作用的潜在下游靶基因：结论：转录因子SOX11在NB中明显上调。结论：转录因子SOX11在NB中明显上调，敲除SOX11可抑制NB细胞SK-N-SH的迁移能力。SOX11可能通过靶向EZH2促进NB的进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Annals of Surgical Treatment and Research SURGERY-

CiteScore

2.30

自引率

7.10%

发文量

期刊介绍： Manuscripts to the Annals of Surgical Treatment and Research (Ann Surg Treat Res) should be written in English according to the instructions for authors. If the details are not described below, the style should follow the Uniform Requirements for Manuscripts Submitted to Biomedical Journals: Writing and Editing for Biomedical Publications available at International Committee of Medical Journal Editors (ICMJE) website (http://www.icmje.org).