Evaluation of Hepatic Glucose and Palmitic Acid Metabolism in Rodents on High-Fat Diet Using Deuterium Metabolic Imaging

IF 3.3 2区医学 Q1 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING

Journal of Magnetic Resonance Imaging Pub Date : 2024-05-09 DOI:10.1002/jmri.29437

Viktoria Ehret MSc, Usevalad Ustsinau MSc, Joachim Friske Mag, Thomas Scherer MD, Clemens Fürnsinn PhD, Thomas H. Helbich MD, Cécile Philippe PhD, Martin Krššák PhD

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引用次数: 0

Abstract

Background

One of the main features of several metabolic disorders is dysregulation of hepatic glucose and lipid metabolism. Deuterium metabolic imaging (DMI) allows for assessing the uptake and breakdown of ²H-labeled substrates, giving specific insight into nutrient processing in healthy and diseased organs. Thus, DMI could be a useful approach for analyzing the differences in liver metabolism of healthy and diseased subjects to gain a deeper understanding of the alterations related to metabolic disorders.

Purpose

Evaluating the feasibility of DMI as a tool for the assessment of metabolic differences in rodents with healthy and fatty livers (FLs).

Study Type

Animal Model.

Population

18 male Sprague Dawley rats on standard (SD, n = 9, healthy) and high-fat diet (HFD, n = 9, FL disease).

Field Strength/Sequence

Phase-encoded 1D pulse-acquire sequence and anatomy co-registered phase-encoded 3D pulse-acquire chemical shift imaging for ²H at 9.4T.

Assessment

Localized and nonlocalized liver spectroscopy was applied at eight time points over 104 minutes post injection. The obtained spectra were preprocessed and quantified using jMRUI (v7.0) and the resulting amplitudes translated to absolute concentration (mM) according to the ²H natural abundance water peak.

Statistical Tests

Two-way repeated measures ANOVA were employed to assess between-group differences, with statistical significance at P < 0.05.

Results

DMI measurements demonstrated no significant difference (P = 0.98) in the uptake of [6,6′-²H₂]glucose between healthy and impaired animals (AUC_SD = 1966.0 ± 151.5 mM - minutes vs. AUC_HFD = 2027.0 ± 167.6 mM·minutes). In the diseased group, the intrahepatic uptake of palmitic acid d-31 was higher (AUC_HFD = 57.4 ± 17.0 mM·minutes, AUC_SD = 33.3 ± 10.5 mM·minutes), but without statistical significance owing to substantial in-group variation (P = 0.73).

Data Conclusion

DMI revealed higher concentrations of palmitic acid in rats with FL disease and no difference in hepatic glucose concentration between healthy and impaired animals. Thus, DMI appears to be a useful tool for evaluating metabolism in rodents with FL disease.

Level of Evidence

Technical Efficacy

Stage 3.

Abstract Image

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利用氘代谢成像评估高脂啮齿动物的肝糖和棕榈酸代谢情况

背景：多种代谢紊乱的主要特征之一是肝脏葡萄糖和脂质代谢失调。氘代谢成像（DMI）可评估 2H 标记底物的摄取和分解，从而具体了解健康器官和患病器官的营养物质处理过程。因此，DMI 可能是分析健康和患病对象肝脏代谢差异的有用方法，从而更深入地了解与代谢紊乱有关的改变。目的：评估将 DMI 作为评估啮齿动物健康肝脏和脂肪肝（FLs）代谢差异的工具的可行性：研究类型：动物模型：18只雄性Sprague Dawley大鼠，分别食用标准饮食（SD，n = 9，健康）和高脂饮食（HFD，n = 9，FL疾病）：场强/序列：9.4T下的相位编码一维脉冲获取序列和解剖共存相位编码三维脉冲获取化学位移成像：在注射后 104 分钟内的八个时间点应用局部和非局部肝脏光谱。使用 jMRUI（v7.0）对获得的光谱进行预处理和量化，并根据 2H 自然丰度水峰将获得的振幅转换为绝对浓度（mM）：采用双向重复测量方差分析来评估组间差异，统计显著性为 P 结果：DMI测量结果显示，健康动物和受损动物对[6,6'-2H2]葡萄糖的摄取无显著差异（P = 0.98）（AUCSD = 1966.0 ± 151.5 mM - 分钟 vs. AUCHFD = 2027.0 ± 167.6 mM - 分钟）。在患病组，棕榈酸 d-31 的肝内摄取量更高（AUCHFD = 57.4 ± 17.0 毫摩尔-分钟，AUCSD = 33.3 ± 10.5 毫摩尔-分钟），但由于组内差异较大，因此没有统计学意义（P = 0.73）：数据结论：DMI 显示患有 FL 疾病的大鼠体内棕榈酸浓度较高，而健康和受损动物的肝糖浓度没有差异。因此，DMI 似乎是评估 FL 疾病啮齿动物新陈代谢的有用工具。

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来源期刊

Journal of Magnetic Resonance Imaging 医学-核医学

CiteScore

9.70

自引率

6.80%

发文量

494

审稿时长

2 months

期刊介绍： The Journal of Magnetic Resonance Imaging (JMRI) is an international journal devoted to the timely publication of basic and clinical research, educational and review articles, and other information related to the diagnostic applications of magnetic resonance.