ICP-MS based detection method combined with Au NP and Ag NP labeling for bacteremia diagnosis

IF 2.6 4区 生物学 Q2 BIOCHEMICAL RESEARCH METHODS
Xiaoting Li , Xin Li , Jiayi Chen , Zuomin Tan , Chuan Wang
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引用次数: 0

Abstract

Bacteremia, as a serious infectious disease, has an increasing incidence and a high mortality rate. Early diagnosis and early treatment are crucial for improving the cure rate. In this work, we proposed an inductively coupled plasma mass spectrometry (ICP-MS)-based detection method combined with gold nanoparticle (Au NP) and silver nanoparticle (Ag NP) labeling for the simultaneous detection of Salmonella and Escherichia coli (E. coli O157:H7) in human blood samples. Salmonella and E. coli O157:H7 were captured by magnetic beads coupled with anti-8G3 and anti-7C2, and then specifically labeled by Au NP-anti-5H12 and Ag NP-anti-8B1 respectively, which were used as signal probes for ICP-MS detection. Under the optimal experimental conditions, the limits of detection of 164 CFU mL−1 for Salmonella, 220 CFU mL−1for E. coli O157:H7 and the linear ranges of 400–80,000 CFU mL−1 Salmonella, 400–60,000 CFU mL−1 E. coli O157:H7 were obtained. The proposed method can realize the simultaneous detection of two types of pathogenic bacteria in human whole blood in 3.5 h, showing great potential for the rapid diagnosis of bacteremia in clinic.

Abstract Image

基于 ICP-MS 的检测方法与 Au NP 和 Ag NP 标记相结合用于菌血症诊断。
菌血症作为一种严重的传染病,发病率越来越高,死亡率也很高。早期诊断和早期治疗对于提高治愈率至关重要。在这项工作中,我们提出了一种基于电感耦合等离子体质谱(ICP-MS)的检测方法,结合金纳米粒子(Au NP)和银纳米粒子(Ag NP)标记,用于同时检测人体血液样本中的沙门氏菌和大肠杆菌(E. coli O157:H7)。沙门氏菌和大肠杆菌O157:H7分别被抗8G3和抗7C2的磁珠捕获,然后分别被Au NP-抗-5H12和Ag NP-抗-8B1特异性标记,作为ICP-MS检测的信号探针。在最佳实验条件下,沙门氏菌的检出限为164 CFU mL-1,大肠杆菌O157:H7的检出限为220 CFU mL-1,线性范围分别为400-80,000 CFU mL-1沙门氏菌和400-60,000 CFU mL-1大肠杆菌O157:H7。该方法可在3.5小时内实现对人体全血中两种致病菌的同时检测,在临床快速诊断菌血症方面具有很大的潜力。
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来源期刊
Analytical biochemistry
Analytical biochemistry 生物-分析化学
CiteScore
5.70
自引率
0.00%
发文量
283
审稿时长
44 days
期刊介绍: The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.
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阿拉丁
sodium citrate
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Bovine serum albumin (BSA)
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