Effect of hydroxyapatite nanoparticles coating of titanium surface on biofilm adhesion: An in vitro study

IF 2.2 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE
Gilda Rocha dos Reis-Neta , Antônio Pedro Ricomini-Filho , Loyse Martorano-Fernandes , Vanessa Felipe Vargas-Moreno , Altair Antoninha Del Bel Cury , Raissa Micaella Marcello-Machado
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引用次数: 0

Abstract

Aim

To evaluate the adhesion of mono and duospecies biofilm on a commercially available dental implant surface coated with hydroxyapatite nanoparticles (nanoHA).

Material and methods

Titanium discs were divided into two groups: double acid-etched (AE) and AE coated with nanoHA (NanoHA). Surface characteristics evaluated were morphology, topography, and wettability. Mono and duospecies biofilms of Streptococcus sanguinis (S. sanguinis) and Candida albicans (C. albicans) were formed. Discs were exposed to fetal bovine serum (FBS) to form the pellicle. Biofilm was growth in RPMI1640 medium with 10% FBS and 10% BHI medium for 6 h. Microbial viability was evaluated using colony-forming unit and metabolic activity by a colorimetric assay of the tetrazolium salt XTT. Biofilm architecture and organization were evaluated by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM).

Results

AE surface had more pores, while NanoHA had even nanoHA crystals distribution. Roughness was similar (AE: 0.59 ± 0.07 µm, NanoHA: 0.69 ± 0.18 µm), but wettability was different (AE: Θw= 81.79 ± 8.55°, NanoHA: Θw= 53.26 ± 11.86°; P = 0.01). NanoHA had lower S. sanguinis viability in monospecies biofilm (P = 0.007). Metabolic activity was similar among all biofilms. In SEM both surfaces on C. albicans biofilm show a similar distribution of hyphae in mono and duospecies biofilms. AE surface has more S. sanguinis than the NanoHA surface in the duospecies biofilm. CLSM showed a large proportion of live cells in all groups.

Conclusions

The nanoHA surface reduced the adhesion of S. sanguinis biofilm but did not alter the adhesion of C. albicans or the biofilm formed by both species.

钛表面羟基磷灰石纳米颗粒涂层对生物膜粘附性的影响:体外研究
材料和方法将钛盘分为两组:双酸蚀(AE)和 AE 涂层纳米HA(NanoHA)。评估的表面特征包括形态、形貌和润湿性。血链球菌(S. sanguinis)和白色念珠菌(C. albicans)形成了单种和双种生物膜。将圆片置于胎牛血清(FBS)中以形成细胞膜。生物膜在含有 10% FBS 和 10% BHI 培养基的 RPMI1640 培养基中生长 6 小时。共焦激光扫描显微镜(CLSM)和扫描电子显微镜(SEM)对生物膜的结构和组织进行了评估。粗糙度相似(AE:0.59 ± 0.07 µm;NanoHA:0.69 ± 0.18 µm),但润湿性不同(AE:Θw= 81.79 ± 8.55°;NanoHA:Θw= 53.26 ± 11.86°;P = 0.01)。NanoHA 在单菌种生物膜中的 S. sanguinis 存活率较低(P = 0.007)。所有生物膜的代谢活性相似。在扫描电子显微镜下,白僵菌生物膜的两个表面在单种和双种生物膜中的菌丝分布相似。在双种生物膜中,AE 表面比 NanoHA 表面有更多的 S. sanguinis。结论 纳米HA 表面减少了 S. sanguinis 生物膜的粘附力,但没有改变白僵菌的粘附力或这两种菌形成的生物膜。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Archives of oral biology
Archives of oral biology 医学-牙科与口腔外科
CiteScore
5.10
自引率
3.30%
发文量
177
审稿时长
26 days
期刊介绍: Archives of Oral Biology is an international journal which aims to publish papers of the highest scientific quality in the oral and craniofacial sciences. The journal is particularly interested in research which advances knowledge in the mechanisms of craniofacial development and disease, including: Cell and molecular biology Molecular genetics Immunology Pathogenesis Cellular microbiology Embryology Syndromology Forensic dentistry
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