Yi-Shen-Hua-Shi regulates intestinal microbiota dysbiosis and protects against proteinuria in patients with chronic kidney disease: a randomized controlled study.

IF 3.9 3区 医学 Q1 MEDICAL LABORATORY TECHNOLOGY
Pharmaceutical Biology Pub Date : 2024-12-01 Epub Date: 2024-05-09 DOI:10.1080/13880209.2024.2345080
Xingtong Dong, Jialing Zhang, Wen Li, Yinping Li, Linpei Jia, Zhaohui Liu, Wenjing Fu, Aihua Zhang
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引用次数: 0

Abstract

Context: Yi-Shen-Hua-Shi (YSHS) is a traditional Chinese medicine that treats chronic kidney disease (CKD). However, its efficacy in reducing proteinuria and underlying mechanisms is unknown.

Objective: This single-center randomized controlled trial explored whether YSHS could improve proteinuria and modulate the gut microbiota.

Materials and methods: 120 CKD patients were enrolled and randomized to receive the renin-angiotensin-aldosterone system (RAAS) inhibitor plus YSHS (n = 56) or RAAS inhibitor (n = 47) alone for 4 months, and 103 patients completed the study. We collected baseline and follow-up fecal samples and clinical outcomes from participants. Total bacterial DNA was extracted, and the fecal microbiome was analyzed using bioinformatics.

Results: Patients in the intervention group had a significantly higher decrease in 24-h proteinuria. After 4 months of the YSHS intervention, the relative abundance of bacteria that have beneficial effects on the body, such as Faecalibacterium, Lachnospiraceae, Lachnoclostridium, and Sutterella increased significantly, while pathogenic bacteria such as the Eggerthella and Clostridium innocuum group decreased. However, we could not find these changes in the control group. Redundancy analysis showed that the decline in 24-h proteinuria during follow-up was significantly correlated with various taxa of gut bacteria, such as Lachnospiraceae and the Lachnoclostridium genus in the YSHS group. KEGG analysis also showed the potential role of YSHS in regulating glycan, lipid, and vitamin metabolism.

Discussion and conclusion: The YSHS granule reduced proteinuria associated with mitigating intestinal microbiota dysbiosis in CKD patients. The definite mechanisms of YSHS to improve proteinuria need to be further explored.

Trial registration: ChiCTR2300076136, retrospectively registered.

益生菌调节慢性肾脏病患者肠道微生物群失调并防止蛋白尿:一项随机对照研究。
背景:益肾化纤丸(YSHS)是一种治疗慢性肾病(CKD)的传统中药,但其降低蛋白尿的疗效及其机制尚不清楚。然而,其降低蛋白尿的疗效和潜在机制尚不清楚:材料与方法:120 名 CKD 患者入组,随机接受肾素-血管紧张素-醛固酮系统(RAAS)抑制剂加用养生堂(56 人)或单用 RAAS 抑制剂(47 人)4 个月,103 名患者完成了研究。我们收集了参与者的基线和随访粪便样本以及临床结果。我们提取了细菌DNA总量,并利用生物信息学分析了粪便微生物组:结果:干预组患者的 24 小时蛋白尿明显减少。经过 4 个月的 "YSHS "干预后,对身体有益的细菌,如粪杆菌属(Faecalibacterium)、拉克诺斯皮拉菌科(Lachnospiraceae)、拉克诺斯皮拉菌属(Lachnoclostridium)和沙氏菌属(Sutterella)的相对丰度明显增加,而致病菌,如埃格特氏菌(Eggerthella)和无核梭菌(Clostridium innocuum)则有所减少。然而,我们在对照组中没有发现这些变化。冗余分析表明,随访期间 24 小时蛋白尿的下降与肠道细菌的不同类群有明显的相关性,如 YSHS 组中的 Lachnospiraceae 和 Lachnoclostridium 属。KEGG分析还显示了YSHS在调节糖、脂质和维生素代谢中的潜在作用:YSHS颗粒能减少蛋白尿,这与缓解CKD患者肠道微生物群失调有关。YSHS改善蛋白尿的确切机制有待进一步探索:ChiCTR2300076136,回顾性注册。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Pharmaceutical Biology
Pharmaceutical Biology 医学-药学
CiteScore
6.70
自引率
2.60%
发文量
191
审稿时长
1 months
期刊介绍: Pharmaceutical Biology will publish manuscripts describing the discovery, methods for discovery, description, analysis characterization, and production/isolation (including sources and surveys) of biologically-active chemicals or other substances, drugs, pharmaceutical products, or preparations utilized in systems of traditional medicine. Topics may generally encompass any facet of natural product research related to pharmaceutical biology. Papers dealing with agents or topics related to natural product drugs are also appropriate (e.g., semi-synthetic derivatives). Manuscripts will be published as reviews, perspectives, regular research articles, and short communications. The primary criteria for acceptance and publication are scientific rigor and potential to advance the field.
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