Ex vivo Anti-Senescence Activity of N-Acetylcysteine in Visceral Adipose Tissue of Obese Volunteers.

IF 3.9 3区医学 Q2 ENDOCRINOLOGY & METABOLISM

Obesity Facts Pub Date : 2024-01-01 Epub Date: 2024-05-10 DOI:10.1159/000539255

Diba Behtaj, Arman Ghorbani, Ghazaleh Eslamian, Nasser Malekpour Alamdari, Maryam Abbasi, Hamid Zand, Azam Shakery, Ghazaleh Shimi, Mohammad Hasan Sohouli, Sepideh Fazeli Taherian

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引用次数: 0

Abstract

Introduction: Excessive visceral adiposity is known to drive the onset of metabolic derangements, mostly involving oxidative stress, prolonged inflammation, and cellular senescence. N-acetylcysteine (NAC) is a synthetic form of l-cysteine with potential antioxidant, anti-inflammatory, and anti-senescence properties. This ex-vivo study aimed to determine the effect of NAC on some markers of senescence including β-galactosidase activity and p16, p53, p21, IL-6, and TNF-α gene expressions in visceral adipose tissue in obese adults.

Methods: This ex-vivo experimental study involved 10 obese participants who were candidates for bariatric surgery. Duplicate biopsies from the abdominal visceral adipose tissue were obtained from the omentum. The biopsies were treated with or without NAC (5 and 10 mm). To evaluate adipose tissue senescence, beta-galactosidase (β-gal) activity and the expression of P16, P21, P53, IL-6, and TNF-α were determined. ANOVA test was employed to analyze the varying markers of cellular senescence and inflammation between treatment groups.

Results: The NAC at concentrations of 5 mm and 10 mm resulted in a noteworthy reduction β-gal activity compared to the control group (p < 0.001). Additionally, the expression of P16, P21, and IL-6 was significantly reduced following treatment with NAC (5 mm) and NAC (10 mm) compared to the control group (All p < 0.001).

Discussion/conclusion: Taken together, these data suggest the senotherapeutic effect of NAC, as it effectively reduces the activity of SA-β-gal and the expression of IL-6, P16, and P21 genes in the visceral adipose tissue of obese individuals.

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肥胖志愿者内脏脂肪组织中的 N-乙酰半胱氨酸体内外抗衰老活性。

导言：众所周知，内脏脂肪过多会导致新陈代谢失调，主要涉及氧化应激、长期炎症和细胞衰老。N- 乙酰半胱氨酸（NAC）是一种 L-半胱氨酸的合成形式，具有潜在的抗氧化、抗炎和抗衰老特性。这项体内外研究旨在确定 NAC 对一些衰老标志物的影响，包括肥胖成年人内脏脂肪组织中的β-半乳糖苷酶活性和 p16、p53、p21、IL-6 和 TNF-α 基因表达：这项体内外实验研究涉及 10 名肥胖者，他们都是减肥手术的候选者。研究人员从网膜处获取了腹部内脏脂肪组织的重复活检样本。活检组织分别接受或不接受 NAC（5 毫摩尔和 10 毫摩尔）处理。为了评估脂肪组织的衰老，测定了β-半乳糖苷酶（β-gal）活性和P16、P21、P53、IL-6和TNF-α的表达。采用方差分析来分析处理组间细胞衰老和炎症标志物的变化：结果：与对照组相比，5 mM 和 10 mM 浓度的 NAC 显著降低了 β-gal 活性（P < 0.001）。此外，与对照组相比，经 NAC（5 mM）和 NAC（10 mM）处理后，P16、P21 和 IL-6 的表达明显减少（均为 P<0.001）：综上所述，这些数据表明 NAC 具有衰老治疗作用，因为它能有效降低肥胖者内脏脂肪组织中 SA-β-gal 的活性以及 IL-6、P16 和 P21 基因的表达。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Obesity Facts 医学-内分泌学与代谢

CiteScore

6.80

自引率

5.60%

发文量

审稿时长

6-12 weeks

期刊介绍： ''Obesity Facts'' publishes articles covering all aspects of obesity, in particular epidemiology, etiology and pathogenesis, treatment, and the prevention of adiposity. As obesity is related to many disease processes, the journal is also dedicated to all topics pertaining to comorbidity and covers psychological and sociocultural aspects as well as influences of nutrition and exercise on body weight. The editors carefully select papers to present only the most recent findings in clinical practice and research. All professionals concerned with obesity issues will find this journal a most valuable update to keep them abreast of the latest scientific developments.