R. G. Sakhabeev, D. S. Polyakov, E. S. Sinitsyna, V. A. Korzhikov-Vlakh, I. O. Bagaeva, E. G. Korzhikova-Vlakh, T. P. Ses, V. S. Tereshina, M. M. Shavlovsky
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引用次数: 0
Abstract
The study is aimed at evaluating the influence of composition
and size of micro- and nanoparticles (MPs and NPs) on the immunogenicity
of protein antigen associated with them. For comparative analysis,
MPs and NPs based on poly(lactic acid) (PLA) and block copolymer
of poly(ethylene glycol) with poly(lactic acid) (PEG-b-PLA) were obtained. Recombinant
human beta2-microglobulin fusion protein with superfolder green
fluorescent protein (β2M-sfGFP) was used for covalent modification
of all types of polymer particles. Immobilization of the model protein
β2M-sfGFP was carried out on the surface of the particles through
the reaction of activated esters with the amino groups of the protein. Immunization
of mice using complex antigen (β2M-sfGFP protein immobilized on
the surface of MPs and NPs of different compositions) was carried
out in four steps. Immunogenicity was assessed by the level of specific
antibodies to sfGFP using enzyme-linked immunoassay. The results
showed a significant increase in antibody levels in the control
groups, which were immunized with a mixture of model protein and
particles of different nature and sizes, compared to the experimental
groups, which were immunized with conjugates of the corresponding
particles with model protein. In the experimental groups, the highest
number of specific antibodies was detected in the case of immunization
of mice with the conjugate of protein and PLA or PEG-b-PLA-based NPs. The introduction
of PEG block into the PLA composition did not significantly affect
the immunogenicity of the protein, while the particle size was of significant
importance. Protein bound to PLA- or PEG-b-PLA-based
NPs showed higher immunogenicity compared protein bound to MPs of
the same compositions, which can be used for practical purposes
to develop vaccines (NP-protein) or “trapping systems” (MP-protein)
that bind infiltrating viruses.
期刊介绍：
Journal of Evolutionary Biochemistry and Physiology publishes original experimental and theoretical and review articles related to evolution of the main forms of metabolism in connection with life origin; comparative and ontogenetic physiology and biochemistry, biochemical evolution of animal world; as well as evolution of functions; morphology, pharmacology, pathophysiology and ecological physiology. The journal welcomes manuscripts from all countries in the English or Russian language.
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