S. E. Belova, I. Y. Oshkin, K. K. Miroshnikov, N. E. Suzina, O. V. Danilova, S. N. Dedysh
{"title":"Methylocapsa polymorpha sp. nov., a Novel Dinitrogen-Fixing Methanotroph from a Subarctic Wetland","authors":"S. E. Belova, I. Y. Oshkin, K. K. Miroshnikov, N. E. Suzina, O. V. Danilova, S. N. Dedysh","doi":"10.1134/s0026261723603949","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>A novel isolate of aerobic, mildly acidophilic methanotrophic bacteria, strain RX1<sup>T</sup>, was obtained from a subarctic <i>Sphagnum</i> peat bog in European Russia. Strain RX1<sup>T</sup> was represented by highly polymorphic, gram-negative, non-motile, encapsulated cells that possessed a particulate methane monooxygenase enzyme and grew on methane, methanol and acetate. Electron microscopy of ultrathin cell sections revealed stacks of intracytoplasmic membrane vesicles located on one side of a cell as characteristic for methanotrophs of the genus <i>Methylocapsa</i>. Strain RX1<sup>T</sup> was capable of fixing dinitrogen, belonged to the family <i>Beijerinckiaceae,</i> and was most closely related to the obligate methanotroph <i>Methylocapsa acidiphila</i> B2<sup>T</sup> (98.8% 16S rRNA gene sequence similarity and 92.2% of PmoA sequence identity). The genome of strain RX1<sup>T</sup> consisted of a 4.02 Mb chromosome and a 291 kb plasmid, and contained one <i>rrn</i> operon, one <i>pmoCAB</i> operon and about 3800 protein-coding genes. The average nucleotide identity between the genomes of strain RX1<sup>T</sup> and <i>M. acidiphila</i> B2<sup>T</sup> was 80.0%. Based on the distinct cell morphology, phenotypic and genotypic differences between strain RX<sup>T</sup> and earlier described members of the genus <i>Methylocapsa</i>, we propose to classify it as representing a novel species of this genus, <i>M. polymorpha</i>, with the type strain RX1<sup>T</sup> = VKM B-3754<sup>T</sup> = UQM 41806<sup>T</sup>.</p>","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":"38 1","pages":""},"PeriodicalIF":1.3000,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s0026261723603949","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
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Abstract
A novel isolate of aerobic, mildly acidophilic methanotrophic bacteria, strain RX1T, was obtained from a subarctic Sphagnum peat bog in European Russia. Strain RX1T was represented by highly polymorphic, gram-negative, non-motile, encapsulated cells that possessed a particulate methane monooxygenase enzyme and grew on methane, methanol and acetate. Electron microscopy of ultrathin cell sections revealed stacks of intracytoplasmic membrane vesicles located on one side of a cell as characteristic for methanotrophs of the genus Methylocapsa. Strain RX1T was capable of fixing dinitrogen, belonged to the family Beijerinckiaceae, and was most closely related to the obligate methanotroph Methylocapsa acidiphila B2T (98.8% 16S rRNA gene sequence similarity and 92.2% of PmoA sequence identity). The genome of strain RX1T consisted of a 4.02 Mb chromosome and a 291 kb plasmid, and contained one rrn operon, one pmoCAB operon and about 3800 protein-coding genes. The average nucleotide identity between the genomes of strain RX1T and M. acidiphila B2T was 80.0%. Based on the distinct cell morphology, phenotypic and genotypic differences between strain RXT and earlier described members of the genus Methylocapsa, we propose to classify it as representing a novel species of this genus, M. polymorpha, with the type strain RX1T = VKM B-3754T = UQM 41806T.
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Microbiology is an is an international peer reviewed journal that covers a wide range of problems in the areas of fundamental and applied microbiology. The journal publishes experimental and theoretical papers, reviews on modern trends in different fields of microbiological science, and short communications with descriptions of unusual observations. The journal welcomes manuscripts from all countries in the English or Russian language.
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