S. E. Belova, I. Y. Oshkin, K. K. Miroshnikov, N. E. Suzina, O. V. Danilova, S. N. Dedysh
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引用次数: 0
Abstract
A novel isolate of aerobic, mildly acidophilic methanotrophic bacteria, strain RX1T, was obtained from a subarctic Sphagnum peat bog in European Russia. Strain RX1T was represented by highly polymorphic, gram-negative, non-motile, encapsulated cells that possessed a particulate methane monooxygenase enzyme and grew on methane, methanol and acetate. Electron microscopy of ultrathin cell sections revealed stacks of intracytoplasmic membrane vesicles located on one side of a cell as characteristic for methanotrophs of the genus Methylocapsa. Strain RX1T was capable of fixing dinitrogen, belonged to the family Beijerinckiaceae, and was most closely related to the obligate methanotroph Methylocapsa acidiphila B2T (98.8% 16S rRNA gene sequence similarity and 92.2% of PmoA sequence identity). The genome of strain RX1T consisted of a 4.02 Mb chromosome and a 291 kb plasmid, and contained one rrn operon, one pmoCAB operon and about 3800 protein-coding genes. The average nucleotide identity between the genomes of strain RX1T and M. acidiphila B2T was 80.0%. Based on the distinct cell morphology, phenotypic and genotypic differences between strain RXT and earlier described members of the genus Methylocapsa, we propose to classify it as representing a novel species of this genus, M. polymorpha, with the type strain RX1T = VKM B-3754T = UQM 41806T.
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Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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