Alexander A. Ukraintsev, Mikhail M. Kutuzov, Olga I. Lavrik
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引用次数: 0
Abstract
Chromatin is an epigenetic platform for implementation of DNA-dependent processes. Nucleosome, as a basic level of chromatin compaction, largely determines its properties and structure. In the study of nucleosomes structure and functions physicochemical tools are actively used, such as magnetic and optical “tweezers”, “DNA curtains”, nuclear magnetic resonance, X-ray crystallography, and cryogenic electron microscopy, as well as optical methods based on Förster resonance energy transfer. Despite the fact that these approaches make it possible to determine a wide range of structural and functional characteristics of chromatin and nucleosomes with high spatial and time resolution, atomic force microscopy (AFM) complements the capabilities of these methods. The results of structural studies of nucleosome focusing on the AFM method development are presented in this review. The possibilities of AFM are considered in the context of application of other physicochemical approaches.
摘要 染色质是实现 DNA 依赖过程的表观遗传平台。核小体作为染色质的基本组成层次，在很大程度上决定了染色质的性质和结构。在核小体结构和功能的研究中，物理化学工具得到了积极的应用，如磁性和光学 "镊子"、"DNA帘子"、核磁共振、X射线晶体学和低温电子显微镜，以及基于佛斯特共振能量转移的光学方法。尽管这些方法能够以较高的空间和时间分辨率确定染色质和核小体的各种结构和功能特征，但原子力显微镜（AFM）是对这些方法能力的补充。本综述介绍了以原子力显微镜方法开发为重点的核小体结构研究结果。本文还结合其他物理化学方法的应用，探讨了原子力显微镜的可能性。
期刊介绍：
Biochemistry (Moscow) is the journal that includes research papers in all fields of biochemistry as well as biochemical aspects of molecular biology, bioorganic chemistry, microbiology, immunology, physiology, and biomedical sciences. Coverage also extends to new experimental methods in biochemistry, theoretical contributions of biochemical importance, reviews of contemporary biochemical topics, and mini-reviews (News in Biochemistry).
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