Continuous sheathless particle separation in viscoelastic fluids with different rheological properties

IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION
Chong-Shan Gan, Zhuang-Zhuang Tian, Lv Liu, Liang-Liang Fan, Liang Zhao
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Abstract

The separation of particles such as cells and bacteria in viscoelastic fluids has significant applications in biomedical fields. At present, one of the main challenges that limit the application of microfluidic technology is to separate particles in the viscoelastic fluids with different rheological properties. For instance, most existing microfluidic devices can only work in the fluid with a specific rheological property, resulting in the requirement of time-consuming design, manufacturing, testing, and optimization of different devices to separate particles in the fluids with different rheological properties. In this work, a novel hybrid three-stage microfluidic device that was made up of a micropore structure and two gradually contracted microchannels was designed to achieve efficient continuous separation of particles in the viscoelastic fluid over a wide range of rheological properties (0.07 < El < 340.41). Different separation strategies including first focusing, then initial separation, and then precise separation (FISPS) and initial separation and then precise separation (ISPS) were found. The separation strategy ISPS occurred at El < 0.14 while the separation strategy FISPS occurred at El > 8.43. In addition, the transformation of the separation mechanism from ISPS to FISPS was found under different flow conditions in the fluid with the transitional rheological properties (0.21 < El < 1.10). The effect of the flow rate and the rheological property of the fluid on microparticle separation were systematically studied by the experiment. With simple structure, easy operation, high separation efficiency, the present microfluidic device would have great potentials in the biomedical and clinical applications, such as the separation of cells for different patients.

Abstract Image

在具有不同流变特性的粘弹性流体中进行连续无鞘颗粒分离
在粘弹性流体中分离细胞和细菌等微粒在生物医学领域有着重要的应用。目前,限制微流控技术应用的主要挑战之一是如何在具有不同流变特性的粘弹性流体中分离颗粒。例如,现有的大多数微流控装置只能在具有特定流变特性的流体中工作,因此需要耗时设计、制造、测试和优化不同的装置,以分离具有不同流变特性的流体中的颗粒。本研究设计了一种新型混合三级微流控装置,由一个微孔结构和两个逐渐收缩的微通道组成,可在流变特性(0.07 < El < 340.41)的宽范围内实现粘弹性流体中颗粒的高效连续分离。研究发现了不同的分离策略,包括先聚焦、再初始分离、再精确分离(FISPS)和先初始分离、再精确分离(ISPS)。分离策略 ISPS 发生在 El < 0.14 时,而分离策略 FISPS 发生在 El > 8.43 时。此外,在具有过渡流变特性(0.21 < El <1.10)的流体中,在不同的流动条件下发现了分离机制从 ISPS 到 FISPS 的转变。实验系统地研究了流速和流体流变特性对微粒分离的影响。该微流控装置结构简单、操作方便、分离效率高,在生物医学和临床应用方面具有很大的潜力,如为不同患者分离细胞。
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来源期刊
Microfluidics and Nanofluidics
Microfluidics and Nanofluidics 工程技术-纳米科技
CiteScore
4.80
自引率
3.60%
发文量
97
审稿时长
2 months
期刊介绍: Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include: 1.000 Fundamental principles of micro- and nanoscale phenomena like, flow, mass transport and reactions 3.000 Theoretical models and numerical simulation with experimental and/or analytical proof 4.000 Novel measurement & characterization technologies 5.000 Devices (actuators and sensors) 6.000 New unit-operations for dedicated microfluidic platforms 7.000 Lab-on-a-Chip applications 8.000 Microfabrication technologies and materials Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).
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