Conyza canadensis, a new host of Tobacco leaf curl Yunnan virus in China

IF 1.1 4区农林科学 Q3 PLANT SCIENCES

Journal of Phytopathology Pub Date : 2024-05-07 DOI:10.1111/jph.13312

Mengdan Du, Hailin Yang, Xueru Song, Zhenggang Li, Xiaoman She, Lin Yu, Guobing Lan, Shanwen Ding, Zifu He, Yafei Tang

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引用次数: 0

Abstract

In April 2023, leaf curl, crinkling symptoms were found on Conyza canadensis plants around tobacco fields located at Yuxi, Yunan province, China. PCR detection with genus-specific primers showed that symptomatic C. canadensis plants was infected by begomovirus. The full-length viral components and satellite molecules from the diseased C. canadensis (YN-2023) were obtained by enrichment of circular DNA with rolling circle amplification, restriction enzyme digestion, cloning, and DNA sequencing. The viral component (2, 750 nt, OR509733) shared the highest nt identity at 92.4% to tobacco leaf curl Yunnan virus isolate YN5741 (TbLCYNV-YN5741 [CN:Yn:Age:16], MN218671). A satellite molecule (1, 339 nt, OR509734) shared the highest nt identity at 86.5% to tobacco curly shoot betasatellite isolate Y115 (TbCSB-Y115 [CN:Yn:03], AJ457822). Another satellite molecule (1, 357 nt, OR509735) shared the highest nt identity at 94.2% to ageratum yellow vein Singapore alphasatellite (AYVSGA-[Singapore:1998], NC003414). Based on the threshold for classification of Begomovirus, Betasatellite, Alphasatellite, YN-2023 was a new strain of TbLCYNV and associated with TbCSB and AYVSGA. To our best knowledge, this is the first report that C. canadensis is a natural host of TbLCYNV.

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中国烟草卷叶病毒的新宿主云南芋

2023 年 4 月，中国云南省玉溪市烟田附近的烟草植株出现卷叶、皱缩症状。利用种属特异性引物进行的 PCR 检测表明，出现症状的烟草植株感染了乞蛾病毒。通过滚圆扩增富集环状DNA、限制性酶消化、克隆和DNA测序，获得了病株（YN-2023）的全长病毒成分和卫星分子。病毒成分（2，750 nt，OR509733）与烟草卷叶云南病毒分离株 YN5741（TbLCYNV-YN5741 [CN:Yn:Age:16]，MN218671）的 nt 一致度最高，达 92.4%。一个卫星分子（1，339 nt，OR509734）与烟草卷曲芽β卫星分离物 Y115（TbCSB-Y115 [CN:Yn:03], AJ457822）的 nt 相同度最高，达 86.5%。另一个卫星分子（1，357 nt，OR509735）与ageratum黄脉新加坡优势卫星（AYVSGA-[Singapore:1998]，NC003414）的最高nt同一性为94.2%。根据 "Begomovirus"、"Betaasatellite"、"Alphasatellite "的分类阈值，YN-2023 是 TbLCYNV 的新毒株，与 TbCSB 和 AYVSGA 相关。据我们所知，这是首次报道 C. canadensis 是 TbLCYNV 的天然宿主。

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来源期刊

Journal of Phytopathology 生物-植物科学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays. Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes. Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.