Comparison of different lateral flow assays on bronchoalveolar lavage fluid for invasive aspergillosis screening in non-hematological patients

IF 2.2 4区医学 Q3 MYCOLOGY

Journal de mycologie medicale Pub Date : 2024-04-30 DOI:10.1016/j.mycmed.2024.101481

Eliane Devillers , Emeline Scherer , Jean-Chistophe Navellou , Frédéric Grenouillet , Laurence Millon , Anne-Pauline Bellanger

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Abstract

Several lateral flow assays (LFA) capable of detecting Aspergillus fumigatus in serum and broncho-alveolar lavage fluid (BALF) within the hour, thereby potentially accelerating the screening process, are now commercially available. We prospectively compared three LFA targeting A. fumigatus on BALF collected from non-surgical intensive care patients between June 2022 and February 2023. The three LFA tested were Sõna Aspergillus galactomannan LFA (Immy), Fungadia Aspergillus antigen (Gadia), and AspLFD (OLM Diagnostics). We compared the results of these LFA with those of the galactomannan (GM) Platelia

Aspergillus enzyme immunoassay (Bio-Rad), culture on Sabouraud medium and Aspergillus qPCR. We tested 97 BALF samples from 92 patients. In total 84 BALF samples tested negative with all three LFA, and four BALF samples tested positive with the AspLFD assay only (OLM). Only one BALF sample tested positive with the three LFA. In addition, three BALF samples tested positive only with the GM Platelia immunoassay. Four diagnosis of probable invasive aspergillosis were retained for the 92 patients tested. This prospective series included very few positive samples. From a practical point of view, the LFA from OLM presented the simplest protocol for use.

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比较不同的支气管肺泡灌洗液侧流检测法，以筛查非血液病患者的侵袭性曲霉菌病

目前市场上有几种侧流检测法（LFA）能够在一小时内检测出血清和支气管肺泡灌洗液（BALF）中的曲霉菌，从而加快筛查过程。我们前瞻性地比较了 2022 年 6 月至 2023 年 2 月期间从非手术重症监护患者处收集的支气管肺泡灌洗液中针对烟曲霉菌的三种 LFA。测试的三种 LFA 分别是 Sõna 曲霉半乳甘露聚糖 LFA（Immy）、Fungadia 曲霉抗原（Gadia）和 AspLFD（OLM Diagnostics）。我们将这些 LFA 的结果与半乳甘露聚糖（GM）Platelia-Aspergillus 酶联免疫分析法（Bio-Rad）、沙保律培养基培养和曲霉 qPCR 的结果进行了比较。我们检测了 92 名患者的 97 份 BALF 样本。共有 84 份 BALF 样本在三种 LFA 检测中均呈阴性，4 份 BALF 样本仅在 AspLFD 检测（OLM）中呈阳性。只有一份 BALF 样本在三种 LFA 检测中均呈阳性。此外，三份 BALF 样本仅在 GM Platelia 免疫测定中呈阳性。在接受检测的 92 名患者中，有 4 人被诊断为可能的侵袭性曲霉菌病。这一系列前瞻性检测中只有极少数样本呈阳性。从实用角度来看，OLM 的 LFA 是最简单的使用方案。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal de mycologie medicale 医学-真菌学

CiteScore

5.10

自引率

2.80%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal de Mycologie Medicale / Journal of Medical Mycology (JMM) publishes in English works dealing with human and animal mycology. The subjects treated are focused in particular on clinical, diagnostic, epidemiological, immunological, medical, pathological, preventive or therapeutic aspects of mycoses. Also covered are basic aspects linked primarily with morphology (electronic and photonic microscopy), physiology, biochemistry, cellular and molecular biology, immunochemistry, genetics, taxonomy or phylogeny of pathogenic or opportunistic fungi and actinomycetes in humans or animals. Studies of natural products showing inhibitory activity against pathogenic fungi cannot be considered without chemical characterization and identification of the compounds responsible for the inhibitory activity. JMM publishes (guest) editorials, original articles, reviews (and minireviews), case reports, technical notes, letters to the editor and information. Only clinical cases with real originality (new species, new clinical present action, new geographical localization, etc.), and fully documented (identification methods, results, etc.), will be considered. Under no circumstances does the journal guarantee publication before the editorial board makes its final decision. The journal is indexed in the main international databases and is accessible worldwide through the ScienceDirect and ClinicalKey platforms.

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