Evaluation of inflammasomes as biomarker following non-surgical periodontal treatment

IF 2.2 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE
Bilge Kabacaoğlu , Hafize Öztürk Özener
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引用次数: 0

Abstract

Objective

The purpose of this study was to investigate interleukin (IL)-1β, IL-18, nod-like receptor pyrin domain-containing protein 3 (NLRP3), apoptosis-related speck-like protein containing a caspase activation and recruitment domain (ASC), and caspase-1 levels in saliva and serum in different periodontal diseases and to evaluate the changes after non-surgical periodontal treatment (NSPT).

Design

A total of 45 participants, 15 healthy, 15 gingivitis, and 15 stage III grade C (SIIIGC) periodontitis patients, were included in the study. Periodontal parameters were assessed, and salivary and serum samples were collected at baseline in all groups and one and three months after NSPT in gingivitis and periodontitis groups. An enzyme-linked immunosorbent assay was used to analyse IL-1β, IL-18, NLRP3, ASC, and caspase-1 levels.

Results

After NSPT, improvement was observed in all clinical parameters, along with periodontal inflamed surface area (PISA) in gingivitis and periodontitis groups. PISA scores were positively correlated with IL-1β, NLRP3, and caspase-1 at baseline (p < 0.05). Salivary and serum IL-1β, NLRP3 levels were higher in periodontitis compared to healthy controls at baseline and reduced after treatment (p < 0.05). Receiver operating characteristic analysis revealed that salivary IL-1β, NLRP3, and caspase-1 had the ability to discriminate SIIIGC periodontitis patients from healthy subjects (p < 0.05).

Conclusion

In conclusion, salivary IL-1β, NLRP3, and caspase-1 are at aberrantly high levels in SIIIGC periodontitis and are remarkably decreased following NSPT; these inflammasome biomarkers may show potential utility in diagnosing and monitoring periodontitis.

将炎症小体作为非手术牙周治疗后的生物标志物进行评估
目的 研究不同牙周疾病患者唾液和血清中白细胞介素(IL)-1β、IL-18、类结节受体吡啉结构域含蛋白3(NLRP3)、凋亡相关的含caspase激活和招募结构域的斑点样蛋白(ASC)以及caspase-1的水平,并评估非手术牙周治疗(NSPT)后的变化。设计研究共纳入 45 名参与者,其中包括 15 名健康患者、15 名牙龈炎患者和 15 名 III 期 C 级(SIIIGC)牙周炎患者。研究人员评估了牙周参数,并在所有组别中收集了基线唾液和血清样本,在牙龈炎和牙周炎组别中收集了 NSPT 后 1 个月和 3 个月的唾液和血清样本。采用酶联免疫吸附试验分析了 IL-1β、IL-18、NLRP3、ASC 和 caspase-1 的水平。结果在 NSPT 后,观察到所有临床参数均有改善,牙龈炎组和牙周炎组的牙周炎症表面积(PISA)也有改善。PISA 评分与基线时的 IL-1β、NLRP3 和 caspase-1 呈正相关(p < 0.05)。与健康对照组相比,牙周炎患者唾液和血清中的 IL-1β、NLRP3 水平在基线时较高,治疗后有所降低(p < 0.05)。接受者操作特征分析显示,唾液中的IL-1β、NLRP3和caspase-1具有区分SIIIGC牙周炎患者和健康受试者的能力(p < 0.05)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Archives of oral biology
Archives of oral biology 医学-牙科与口腔外科
CiteScore
5.10
自引率
3.30%
发文量
177
审稿时长
26 days
期刊介绍: Archives of Oral Biology is an international journal which aims to publish papers of the highest scientific quality in the oral and craniofacial sciences. The journal is particularly interested in research which advances knowledge in the mechanisms of craniofacial development and disease, including: Cell and molecular biology Molecular genetics Immunology Pathogenesis Cellular microbiology Embryology Syndromology Forensic dentistry
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