A Comprehensive Pathophysiologic, Histologic, and Biochemical Analysis of Buthus paris (C. L. Koch, 1839) Venom.

IF 1.4 4区医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

Wilderness & Environmental Medicine Pub Date : 2024-09-01 Epub Date: 2024-05-07 DOI:10.1177/10806032241249748

Abdessamad Elmourid, My Abdelmonaim Elhidan, Samia Boussaa, Mehdi Ait Laaradia, Btissam Bouimeja, Omar Amahmid, Mohamed Merzouki, Oulaid Touloun

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引用次数: 0

Abstract

Introduction: Buthus species, including B paris, are classified as one of the most dangerous scorpion genera in Morocco, implicated in several cases of human death. Our objective is to characterize, for the first time, the toxicity and histopathologic and biochemical impacts of B paris venom.

Methods: We investigated the experimental pathophysiology of B paris venom by examining histologic changes in vital organs (heart, kidneys, liver, and lungs) and assessing biochemical enzymatic markers (alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, creatine phosphokinase, urea, and creatinine) in mice injected subcutaneously with 2 doses of 400 and 450 mg·kg^-1.

Results: Our results showed that the subcutaneous median lethal dose of B paris venom was around 0.52 mg·kg^-1. Histologic findings revealed significant tissue damage in the previously mentioned vital organs, confirmed through biochemical analysis indicating impaired heart and liver functions. Additionally, an increase in urea, creatinine, and glucose levels occurred following B paris venom injection.

Conclusion: Our findings show that B paris venom exhibits a high level of experimental toxicity. These results highlight the potentially lethal nature of this venom and emphasize the potential medical importance of this species.

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Buthus paris（C. L. Koch，1839 年）毒液的病理生理学、组织学和生化综合分析。

导言：包括B paris在内的Buthus种被列为摩洛哥最危险的蝎属之一，曾多次造成人类死亡。我们的目的是首次描述 B paris 毒液的毒性、组织病理学和生化影响：方法：我们对小鼠皮下注射 400 毫克和 450 毫克-千克-1 两种剂量的 B paris 毒液，通过检查重要器官（心脏、肾脏、肝脏和肺部）的组织学变化和评估生化酶标记物（丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、乳酸脱氢酶、肌酸磷酸激酶、尿素和肌酐），研究了 B paris 毒液的实验病理生理学：结果表明，B paris 毒液皮下注射的中位致死剂量约为 0.52 mg-kg-1。组织学研究结果表明，上述重要器官的组织受到严重破坏，生化分析表明心脏和肝脏功能受损也证实了这一点。此外，在注射 B paris 毒液后，尿素、肌酐和葡萄糖水平也有所上升：我们的研究结果表明，B paris 毒液具有很高的实验毒性。结论：我们的研究结果表明，B paris 毒液具有很高的实验毒性，这些结果突显了该毒液的潜在致命性，并强调了该物种潜在的医学重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Wilderness & Environmental Medicine 医学-公共卫生、环境卫生与职业卫生

CiteScore

2.10

自引率

7.10%

发文量

审稿时长

>12 weeks

期刊介绍： Wilderness & Environmental Medicine, the official journal of the Wilderness Medical Society, is the leading journal for physicians practicing medicine in austere environments. This quarterly journal features articles on all aspects of wilderness medicine, including high altitude and climbing, cold- and heat-related phenomena, natural environmental disasters, immersion and near-drowning, diving, and barotrauma, hazardous plants/animals/insects/marine animals, animal attacks, search and rescue, ethical and legal issues, aeromedial transport, survival physiology, medicine in remote environments, travel medicine, operational medicine, and wilderness trauma management. It presents original research and clinical reports from scientists and practitioners around the globe. WEM invites submissions from authors who want to take advantage of our established publication''s unique scope, wide readership, and international recognition in the field of wilderness medicine. Its readership is a diverse group of medical and outdoor professionals who choose WEM as their primary wilderness medical resource.