Recent Developments of Hybrid Fluorescence Techniques: Advances in Amyloid Detection Methods.

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
A Miraclin Prasanna, Priyankar Sen
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引用次数: 0

Abstract

Amyloid fibrils are formed from various pathological proteins. Monitoring their aggregation process is necessary for early detection and treatment. Among the available detection techniques, fluorescence is simple, intuitive, and convenient due to its sensitive and selective mode of detection. It has certain disadvantages like poor photothermal stability and detection state limitation. Research has focused on minimising the limitation by developing hybrid fluorescence techniques. This review focuses on the two ways fluorescence (intrinsic and extrinsic) has been used to monitor amyloid fibrils. In intrinsic/label free fluorescence: i) The fluorescence emission through aromatic amino acid residues like phenylalanine (F), tyrosine (Y) and tryptophan (W) is present in amyloidogenic peptides/protein sequence. And ii) The structural changes from alpha helix to cross-β-sheet structures during amyloid formation contribute to the fluorescence emission. The second method focuses on the use of extrinsic fluorophores to monitor amyloid fibrils i) organic dyes/small molecules, ii) fluorescent tagged proteins, iii) nanoparticles, iv) metal complexes and v) conjugated polymers. All these fluorophores have their own limitations. Developing them into hybrid fluorescence techniques and converting it into biosensors can contribute to early detection of disease.

混合荧光技术的最新发展:淀粉样蛋白检测方法的进展。
淀粉样蛋白纤维是由各种病理蛋白形成的。监测其聚集过程对于早期检测和治疗非常必要。在现有的检测技术中,荧光检测灵敏度高、选择性强,简单、直观、方便。但它也有一些缺点,如光热稳定性差和检测状态限制。研究的重点是通过开发混合荧光技术来尽量减少这种限制。本综述将重点介绍荧光(内在和外在)用于监测淀粉样蛋白纤维的两种方法。在本征/无标记荧光中:i) 通过芳香族氨基酸残基如苯丙氨酸(F)、酪氨酸(Y)和色氨酸(W)发射的荧光存在于淀粉样蛋白肽/蛋白质序列中。ii) 在淀粉样蛋白形成过程中,从α螺旋结构到交叉β片状结构的结构变化有助于荧光发射。第二种方法侧重于使用外在荧光团来监测淀粉样纤维,即有机染料/小分子、ii) 荧光标记蛋白质、iii) 纳米粒子、iv) 金属复合物和 v) 共轭聚合物。所有这些荧光团都有各自的局限性。将它们开发成混合荧光技术并将其转化为生物传感器,有助于疾病的早期检测。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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