Evaluation of cytotoxicity, acute toxicity, genotoxicity and antioxidant and antigenotoxicity activities of the sarcotesta fraction of punica granatum L. rich in lectin (PgTel).

IF 2.3 4区 医学 Q3 ENVIRONMENTAL SCIENCES
Leila Viviany Araújo Coêlho, Elinaldo Francisco de Lima Bento, Wêndeo Kennedy Costa, Magda Rhayanny Assunção Ferreira, Luiz Alberto Lira Soares, Márcia Vanusa da Silva, Patrícia Maria Guedes Paiva, Alisson Macário de Oliveira, Thiago Henrique Napoleão
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引用次数: 0

Abstract

Punica granatum, popularly known as pomegranate, is a fruit tree with wide worldwide distribution, containing numerous phytochemicals of great medicinal value. The aim of the present study was to determine the phytochemical profile and antioxidant potential of a protein fraction (PF) derived from P. granatum sarcotesta which is rich in lectin. In addition, the acute oral toxicity, genotoxicity and antigenotoxicity of this protein fraction (PF) from P. granatum sarcotesta was measured. The phytochemical profile of PF was determined using HPLC. The in vitro antioxidant effect was assessed using the methods of total antioxidant capacity (TAC) and DPPH and ABTS+ radical scavenging. Acute oral toxicity was determined in female Swiss mice administered a single dose of 2000 mg/kg. This PF was examined for genotoxicity and antigenotoxicity at doses of 500, 1000 and 2000 mg/kg, utilizing mouse peripheral blood cells. Phytochemical characterization detected a high content of ellagic acid and antioxidant capacity similar to that of ascorbic acid (positive control). PF was not toxic (LD50 >2000 mg/kg) and did not exert a genotoxic effect in mice. PF protected the DNA of peripheral blood cells against damage induced by cyclophosphamide. In conclusion, this PF fraction exhibited significant antioxidant activity without initiating toxic or genotoxic responses in mice.

评估富含凝集素(PgTel)的石榴果肉部分的细胞毒性、急性毒性、遗传毒性以及抗氧化和抗原毒性活性。
石榴(Punica granatum),俗称石榴,是一种广泛分布于世界各地的果树,含有多种极具药用价值的植物化学物质。本研究旨在确定从富含凝集素的肉石榴中提取的蛋白质组分(PF)的植物化学成分和抗氧化潜力。此外,还测定了从肉苁蓉中提取的这种蛋白质组分(PF)的急性口服毒性、遗传毒性和抗原毒性。使用高效液相色谱法测定了 PF 的植物化学成分。采用总抗氧化能力(TAC)、DPPH 和 ABTS+ 自由基清除法评估了体外抗氧化效果。对雌性瑞士小鼠进行了急性口服毒性测定,单次剂量为 2000 毫克/千克。在剂量为 500、1000 和 2000 毫克/千克时,利用小鼠外周血细胞对该 PF 的遗传毒性和抗原毒性进行了检测。植物化学特征检测发现,鞣花酸含量较高,抗氧化能力与抗坏血酸(阳性对照)相似。PF 没有毒性(半数致死剂量大于 2000 毫克/千克),也没有对小鼠产生基因毒性作用。PF 能保护外周血细胞的 DNA 免受环磷酰胺的损伤。总之,这种全氟辛烷磺酸馏分具有显著的抗氧化活性,不会对小鼠产生毒性或遗传毒性反应。
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来源期刊
CiteScore
5.20
自引率
19.20%
发文量
46
审稿时长
8-16 weeks
期刊介绍: The Journal of Toxicology and Environmental Health, Part A , Current Issues is an authoritative journal that features strictly refereed original research in the field of environmental sciences, public and occupational health, and toxicology.
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