[Comparison of the microbiota diversity between autogenous and anautogenous Culex pipiens pallens].

Q3 Medicine

中国血吸虫病防治杂志 Pub Date : 2024-04-02 DOI:10.16250/j.32.1374.2023204

J Lei, W Lü, W Wang, H Wang, X Guo, P Cheng, M Gong, L Liu

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引用次数: 0

Abstract

Objective: To investigate the microbiota composition and diversity between autogenous and anautogenous Culex pipiens pallens, so as to provide insights into unraveling the pathogenesis of autogeny in Cx. pipiens pallens.

Methods: Autogenous and anautogenous adult Cx. pipiens pallens samples were collected at 25 ℃, and the hypervariable regions of the microbial 16S ribosomal RNA (16S rRNA) gene was sequenced on the Illumina NovaSeq 6000 sequencing platform. The microbiota abundance and diversity were evaluated using the alpha diversity index, and the difference in the microbiota structure was examined using the beta diversity index. The microbiota with significant differences in the abundance between autogenous and anautogenous adult Cx. pipiens pallens samples was identified using the linear discriminant analysis effect size (LEfSe).

Results: The microbiota in autogenous and anautogenous Cx. pipiens pallens samples belonged to 18 phyla, 28 classes, 70 orders, 113 families, and 170 genera, and the dominant phyla included Proteobacteria, Bacteroidetes, and so on. At the genus level, Wolbachia was a common dominant genus, and the relative abundance was (77.6 ± 11.3)% in autogenous Cx. pipiens pallens samples and (47.5 ± 8.5)% in anautogenous mosquito samples, while Faecalibaculum (0.4% ± 0.1%), Dubosiella (0.5% ± 0.0%) and Massilia (0.5% ± 0.1%) were specific species in autogenous Cx. pipiens pallens samples. Alpha diversity analysis showed that higher Chao1 index and ACE index in autogenous Cx. pipiens pallens samples than in anautogenous samples (both P values > 0.05), and lower Shannon index (P > 0.05) and Simpson index (P < 0.05) in autogenous Cx. pipiens pallens samples than in anautogenous samples. LEfSe analysis showed a total of 48 significantly different taxa between autogenous and anautogenous Cx. pipiens pallens samples (all P values < 0.05).

Conclusions: There is a significant difference in the microbiota diversity between autogenous and anautogenous Cx. pipiens pallens.

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[自体和非自体库蚊微生物群多样性比较]。

目的研究自生和非自生苍喙库蚊的微生物区系组成和多样性，为揭示苍喙库蚊自生的致病机理提供见解：方法：采集 25 ℃下的自源和非自源苍蝇成虫样本，在 Illumina NovaSeq 6000 测序平台上对微生物 16S 核糖体 RNA（16S rRNA）基因的超变区进行测序。使用α多样性指数评估微生物群的丰度和多样性，使用β多样性指数检验微生物群结构的差异。利用线性判别分析效应大小（LEfSe）确定了自源性和非自源性苍蝇成虫样本中丰度存在显著差异的微生物群：自体和非自体苍蝇样本中的微生物群隶属于 18 个门、28 个类、70 个目、113 个科、170 个属，优势菌门包括变形菌门、类杆菌门等。在属一级，Wolbachia 是常见的优势属，其相对丰度在自体苍蝇蚊样本中为 (77.6 ± 11.3)%，在非自体蚊样本中为 (47.5 ± 8.5)%；Faecalibaculum（0.4% ± 0.1%）、Dubosiella（0.5% ± 0.0%）和 Massilia（0.5% ± 0.1%）是自体苍蝇蚊样本中的特异种。α多样性分析表明，自体苍蝇样本的 Chao1 指数和 ACE 指数高于非自体样本（P 值均大于 0.05），而自体苍蝇样本的 Shannon 指数（P 值大于 0.05）和 Simpson 指数（P 值小于 0.05）低于非自体样本。LEFSe分析表明，自生和非自生苍蝇样本中共有48个显著不同的类群（所有P值均小于0.05）：结论：自体和非自体苍蝇库蚊微生物群多样性存在明显差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

中国血吸虫病防治杂志 Medicine-Medicine (all)

CiteScore

1.30

自引率

0.00%

发文量

7021

期刊介绍： Chinese Journal of Schistosomiasis Control (ISSN: 1005-6661, CN: 32-1374/R), founded in 1989, is a technical and scientific journal under the supervision of Jiangsu Provincial Health Commission and organised by Jiangsu Institute of Schistosomiasis Control. It is a scientific and technical journal under the supervision of Jiangsu Provincial Health Commission and sponsored by Jiangsu Institute of Schistosomiasis Prevention and Control. The journal carries out the policy of prevention-oriented, control-oriented, nationwide and grassroots, adheres to the tenet of scientific research service for the prevention and treatment of schistosomiasis and other parasitic diseases, and mainly publishes academic papers reflecting the latest achievements and dynamics of prevention and treatment of schistosomiasis and other parasitic diseases, scientific research and management, etc. The main columns are Guest Contributions, Experts‘ Commentary, Experts’ Perspectives, Experts' Forums, Theses, Prevention and Treatment Research, Experimental Research, The main columns include Guest Contributions, Expert Commentaries, Expert Perspectives, Expert Forums, Treatises, Prevention and Control Studies, Experimental Studies, Clinical Studies, Prevention and Control Experiences, Prevention and Control Management, Reviews, Case Reports, and Information, etc. The journal is a useful reference material for the professional and technical personnel of schistosomiasis and parasitic disease prevention and control research, management workers, and teachers and students of medical schools. 　　 The journal is now included in important domestic databases, such as Chinese Core List (8th edition), China Science Citation Database (Core Edition), China Science and Technology Core Journals (Statistical Source Journals), and is also included in MEDLINE/PubMed, Scopus, EBSCO, Chemical Abstract, Embase, Zoological Record, JSTChina, Ulrichsweb, Western Pacific Region Index Medicus, CABI and other international authoritative databases.