Micro-RNA-371a-3p in Germ Cell Testicular Tumors on Diagnosis: A Prospective Case-Control Study in Turkish Population.

IF 1.5 4区医学 Q3 UROLOGY & NEPHROLOGY

Urology Journal Pub Date : 2024-06-09 DOI:10.22037/uj.v20i.8002

Muzaffer Tansel Kılınç, Yunus Emre Göger, Mehmet Serkan Özkent, Özcan Kılıç, Betül Okur Altındaş, Mahmut Selman Yıldırım, Giray Karalezli

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引用次数: 0

Abstract

Purpose: We aimed to evaluate the diagnostic sensitivity and specificity of the miRNA-371a-3p for the primary diagnosis of germ cell tumors (GCT) and to investigate its relationship with pathological factors and clinical stage in the Turkish population.

Materials and methods: In this prospective study, a total of 60 patients with GCTs, and 40 healthy male controls were examined for serum levels of miRNA-371a-3p before orchiectomy and again two weeks after surgery. The miRNA-371a-3p, alpha-fetoprotein (AFP), and beta-human chorionic gonadotropin (bHCG) levels in the preoperative and postoperative periods were compared at different clinical stages. Receiver operating characteristics curve analyses were performed to determine the sensitivity and specificity of miRNA-371a-3p. Clinical and pathological factors such as clinical stage (CS), tumor size, histology, rete testis invasion, and lymphovascular invasion, potentially impacting miRNA-371a-3p expression levels (relative quantity, RQ), were evaluated statistically.

Results: The sensitivity of miR-371a-3p in GCT patients was 98.3%, and the specificity was 95% (AUC = 0.997 [95%Cl:0.99-1], p < .001). miR-371a-3p expression was not detected in two patients with teratoma. The median miR-371a-3p RQ was 489 times in GCT and 2.2 times in the Control group (p < .001). In the postoperative period, there was a significant decrease in AFP and bHCG levels in all CS-1 (p = .01) and 30% of the other CS (p = .3). Throughout this time there was a decrease of 19 times at the miR-371a-3p RQ in CS-1(p < .001) and 1.6 times in the other CS (p < .001). The miR-371a-3p RQs were correlated with tumor size and CS.

Conclusion: The miR-371a-3p seems to have higher diagnostic accuracy than classical serum tumor markers in GCT.

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诊断时生殖细胞睾丸肿瘤中的微 RNA-371a-3p：土耳其人群中的前瞻性病例对照研究。

目的：我们旨在评估土耳其人群中miRNA-371a-3p对生殖细胞瘤（GCT）初诊的诊断敏感性和特异性，并研究其与病理因素和临床分期的关系：在这项前瞻性研究中，共对 60 名 GCT 患者和 40 名健康男性对照者进行了睾丸切除术前和术后两周的血清 miRNA-371a-3p 水平检测。比较了不同临床阶段术前和术后的 miRNA-371a-3p、甲胎蛋白（AFP）和β-人绒毛膜促性腺激素（bHCG）水平。进行了接收者操作特征曲线分析，以确定 miRNA-371a-3p 的敏感性和特异性。统计评估了可能影响 miRNA-371a-3p 表达水平（相对量，RQ）的临床和病理因素，如临床分期（CS）、肿瘤大小、组织学、睾丸前壁侵犯和淋巴管侵犯：miR-371a-3p在GCT患者中的敏感性为98.3%，特异性为95%（AUC = 0.997 [95%Cl:0.99-1], p < .001）。中位 miR-371a-3p RQ 在 GCT 组为 489 倍，在对照组为 2.2 倍（p < .001）。术后，所有 CS-1 组（p = .01）和 30% 的其他 CS 组（p = .3）的 AFP 和 bHCG 水平均显著下降。在此期间，CS-1 的 miR-371a-3p RQ 下降了 19 倍（p < .001），其他 CS 下降了 1.6 倍（p < .001）。miR-371a-3p RQ与肿瘤大小和CS相关：结论：与传统的血清肿瘤标志物相比，miR-371a-3p 似乎对 GCT 具有更高的诊断准确性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Urology Journal UROLOGY & NEPHROLOGY-

CiteScore

2.60

自引率

6.70%

发文量

审稿时长

6-12 weeks

期刊介绍： As the official journal of the Urology and Nephrology Research Center (UNRC) and the Iranian Urological Association (IUA), Urology Journal is a comprehensive digest of useful information on modern urology. Emphasis is on practical information that reflects the latest diagnostic and treatment techniques. Our objectives are to provide an exceptional source of current and clinically relevant research in the discipline of urology, to reflect the scientific work and progress of our colleagues, and to present the articles in a logical, timely, and concise format that meets the diverse needs of today’s urologist. Urology Journal publishes manuscripts on urology and kidney transplantation, all of which undergo extensive peer review by recognized authorities in the field prior to their acceptance for publication. Accordingly, original articles, case reports, and letters to editor are encouraged.